Abstract | BACKGROUND: METHODS: The peptide fraction from the larvae of Musca domestica L. (MDPF) was evaluated for its adjuvant potentials on the immune responses to ovalbumin (OVA) and avian influenza vaccine (rL-H5) by determining antigen-specific antibody titers, splenocyte proliferation, activity of natural killer (NK) cell, the secretion of cytokines from splenocytes in the immunized mice. RESULTS: MDPF significantly enhanced not only the concanavalin A (Con A)-, lipopolysaccharide (LPS)- and antigen-stimulated splenocyte proliferation, but serum antigen-specific IgG, IgG1, IgG2a, and IgG2b antibody titers in the mice immunized with OVA and rL-H5. MDPF also remarkably promoted the killing activities of NK cells in splenocytes from the mice immunized with rL-H5. Furthermore, MDPF significantly promoted the production of Th1 (IL-2 and IFN-γ) and Th2 (IL-10) cytokines from splenocytes in the immunized mice. CONCLUSIONS: The results indicated that MDPF had a potential to increase both cellular and humoral immune responses and elicit a balanced Th1/Th2 response, and that MDPF may be a safe and efficacious vaccine adjuvant candidate.
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Authors | Liqing Chen, Juan Zhang, Hongxiang Sun |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 15
Issue 1
Pg. 427
(Dec 02 2015)
ISSN: 1472-6882 [Electronic] England |
PMID | 26630909
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Cytokines
- Immunoglobulin G
- Influenza Vaccines
- Peptides
- Tissue Extracts
- Ovalbumin
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Topics |
- Adjuvants, Immunologic
(pharmacology)
- Animals
- Cell Proliferation
- Cytokines
(blood, immunology)
- Female
- Houseflies
- Humans
- Immunoglobulin G
(blood)
- Influenza Vaccines
(immunology)
- K562 Cells
- Killer Cells, Natural
(drug effects, immunology)
- Larva
(chemistry)
- Mice
- Mice, Inbred ICR
- Ovalbumin
(immunology)
- Peptides
(pharmacology)
- Spleen
(cytology, immunology)
- Tissue Extracts
(pharmacology)
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