Abstract | OBJECTIVES: MATERIALS AND METHODS: Forty-four patients with previously untreated clinically late T2 or T3 OSCC of cN0 were evaluated for primary tumors and 166 sentinel lymph nodes (SLNs). Primary tumors were immunohistochemically analyzed for expressions of VEGFs. Densities of lymphatic vessels (LVDpodoplanin) and high endothelial venules (HEVD) in the SLNs were also calculated using antibodies for each marker, podoplanin and MECA-79, respectively. RESULTS: In 25 patients, all lymph nodes were metastasis-negative, whereas, in 19 patients, metastasis was positive for at least one lymph node (either at SLN, non-SLN, or nodal recurrence). From the analyses of 140 SLNs without metastasis, LVDpodoplanin in 50 SLNs of metastasis-positive cases was significantly higher than that in 90 SLNs of metastasis-negative cases (p = 0.0025). HEVD was not associated with lymph node metastasis. The patients with VEGF-A-High or VEGF-D-High tumors had significantly higher LVDpodoplanin than patients with their Low counterparts (p = 0.0233 and p = 0.0209, respectively). In cases with lymph node metastasis, the VEGF-D-expression score was significantly higher than in those without lymph node metastasis (p = 0.0006). CONCLUSIONS:
|
Authors | Naohiro Wakisaka, Yasuhisa Hasegawa, Seiichi Yoshimoto, Kouki Miura, Akihiro Shiotani, Junkichi Yokoyama, Masashi Sugasawa, Makiko Moriyama-Kita, Kazuhira Endo, Tomokazu Yoshizaki |
Journal | PloS one
(PLoS One)
Vol. 10
Issue 12
Pg. e0144056
( 2015)
ISSN: 1932-6203 [Electronic] United States |
PMID | 26630663
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Biomarkers, Tumor
- Neoplasm Proteins
- VEGFA protein, human
- VEGFD protein, human
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factor D
|
Topics |
- Adult
- Aged
- Biomarkers, Tumor
(metabolism)
- Carcinoma, Squamous Cell
(metabolism, pathology)
- Female
- Humans
- Lymphangiogenesis
- Lymphatic Metastasis
- Male
- Middle Aged
- Mouth Neoplasms
(metabolism, pathology)
- Neoplasm Proteins
(metabolism)
- Sentinel Lymph Node Biopsy
- Vascular Endothelial Growth Factor A
(metabolism)
- Vascular Endothelial Growth Factor D
(metabolism)
|