Abstract |
Thiolutin was found to inhibit the utilization of glucose and other growth substrates in Escherichia coli. The inhibition was detected by a sharp drop of the respiration rate after addition of the antibiotic. The actual function affected was allocated to the cytoplasmic membrane of the bacterial cells by the following evidence: --spheroplasts were affected like intact cells, --individual reactions of either the electron transport chain or the glycolytic pathway were not inhibited, -- glucose consumption in the culture stopped and the cells accumulated guanosine tetraphosphate as under starvation conditions, --activation of the cell's apo- glucose dehydrogenase restored respiration via bypassing the glucose phosphotransferase system. It was concluded that the transport of certain substrates across the membrane was inhibited.
|
Authors | R Bergmann |
Journal | Antonie van Leeuwenhoek
(Antonie Van Leeuwenhoek)
Vol. 55
Issue 2
Pg. 143-52
( 1989)
ISSN: 0003-6072 [Print] Netherlands |
PMID | 2662903
(Publication Type: Journal Article)
|
Chemical References |
- Anti-Bacterial Agents
- Culture Media
- Pyrrolidinones
- acetopyrrothine
- Glucose
|
Topics |
- Anti-Bacterial Agents
(pharmacology)
- Cell Membrane
(drug effects, metabolism)
- Culture Media
- Escherichia coli
(drug effects, metabolism, ultrastructure)
- Glucose
(metabolism)
- Oxygen Consumption
(drug effects)
- Pyrrolidinones
(pharmacology)
- Spheroplasts
(drug effects, metabolism)
|