Interferon-λ1 suppresses invasion and enhances autophagy in human osteosarcoma cell.

Abstract

OBJECTIVE:

The purpose of the present study was to determine whether type III IFN can modulate the autophagic response in human osteosarcoma cell.

METHODS:

Human osteosarcoma cell were treated with Interferon-λ1. We investigated that Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells by Matrigel invasion assay. Autophagy were assessed by acridine orange staining, MDC staining and Transmission electron microscopy.

RESULTS:

In this study, we found that Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells. Acridine orange staining and MDC staining showed that Interferon-λ1 triggered the accumulation of acidic vesicular and autolysosomes in osteosarcoma cell. The acridine orange osteosarcoma cell ratios were 3.6 ± 0.5%, 4.5 ± 0.8%, and 12.4 ± 1.7% after treatment with 1, 10, and 100 ng/mL Interferon-λ1 for 48 h. Osteosarcoma cell cells treated with 100 ng/mL Interferon-λ1 for 48 h developed autophagy some-like characteristics, including single or double-membrane vacuoles containing intact and degraded cellular debris.

CONCLUSIONS:

Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells. Autophagy can be induced in a dose-dependent manner by treatment with Interferon-λ1 in osteosarcoma cell.

• Authors: Dongmei Gao, Jun Zhao, Xiuyi Li, Yuanyuan Xia, Yu Cai, Jiwen Pan, Hong Zhou, Yong Fang, Shouzhu Zhang, He Wen
• Journal: International journal of clinical and experimental medicine (Int J Clin Exp Med) Vol. 8 Issue 9 Pg. 14999-5004 ( 2015) ISSN: 1940-5901 [Print] United States
• PMID: 26628983 (Publication Type: Journal Article)