Genetic association between CD44 polymorphisms and chronic hepatitis B virus infection in a Chinese Han population.

Abstract

AIMS:

This article aimed at discussing the association of chronic hepatitis B virus (HBV) infection with CD44 polymorphisms in Chinese Han population; meanwhile, the interaction of polymorphisms was also analyzed based on chronic HBV infection.

METHODS:

The genotyping of CD44 polymorphisms was conducted by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 108 HBV infected and 130 healthy persons. The genotype distributions of CD44 rs187115, rs13347 in the control group were checked by Hardy-Weinberg equilibrium (HWE). The strength of the relevance between polymorphism and disease was measured by odds ratio (OR) with corresponding 95% confidence interval (CI) calculated by χ(2) test. The 2×4 crossover analysis method was used to conduct the interaction analysis of polymorphisms.

RESULTS:

The genotype distributions in controls conformed to HWE. GG genotype and G allele frequencies in rs187115 were obviously higher in cases than the controls (P=0.02, 0.04). Compared with the common genotype CC, individual who carried mutant genotypes (CT and TT) of rs13347 had a significantly high risk to suffer from HBV infection (OR=1.99, P=0.02 for CT; OR=3.56, P=3.00×10(-3) for TT), furthermore, CT+TT genotype also showed a high susceptibility (OR=2.27, P=2.00×10(-3)). Similarly, T allele of rs13347 increased 0.98 times risk in cases compared with controls (OR=1.98, 95% CI=1.34-2.92). The two polymorphisms in CD44 presented a positive interaction.

CONCLUSION:

CD44 polymorphisms are associated with chronic HBV infection as the risk factors, and the synergistic action is also found between the two polymorphisms.

• Authors: Fan Li, Qing Shao, Dong Ji, Bing Li, Guofeng Chen
• Journal: International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 8 Issue 9 Pg. 11675-9 ( 2015) ISSN: 1936-2625 [Electronic] United States
• PMID: 26617909 (Publication Type: Journal Article)

Chemical References

• CD44 protein, human
• Hyaluronan Receptors

Topics

• Adult
• Asian People (genetics)
• Case-Control Studies
• Female
• Genetic Predisposition to Disease (genetics)
• Genotype
• Hepatitis B, Chronic (genetics)
• Humans
• Hyaluronan Receptors (genetics)
• Male
• Middle Aged
• Odds Ratio
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Polymorphism, Single Nucleotide
• Young Adult