Abstract | OBJECTIVE: To determine the rate of occurrence and genotypes of Epstein-Barr Virus (EBV) among pediatric renal and liver transplants recipients. DESIGN: Observational study. SETTING: PARTICIPANTS: 70 pediatric solid organ transplant recipients and 60 voluntary healthy donors. METHODS: Polymerase chain reaction (PCR) for detection and genotyping of EBV were carried out using genes targeting Viral capsid antigen, Nuclear antigen 1, 2 and 3, followed by real time PCR for viral load determination and further confirmed by phylogenetic analysis. RESULTS: EBV was detected in 35 (51.4%) samples (32 liver and 4 renal transplants) with high viral load. Type A was detected in 33 samples, Type B in 2 liver transplant patients, and co-infection in one liver transplant patient who developed Post-transplant Lymphoproliferative Disorder (PTLD). Real time PCR results correlated with conventional PCR. The mean viral load for patients who did not develop PTLD was 50,424 copies/mL. Overall EBV load in patient with PTLD ranged from 1,40,392 copies/mL prior to PTLD diagnosis to 62,124 copies /mL post treatment. CONCLUSION:
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Authors | Madhuravasal Krishnan Janani, Jambulingam Malathi, Mohamed Rela, Mohammed Farouk, J Padmapriya, Hajib N Madhavan |
Journal | Indian pediatrics
(Indian Pediatr)
Vol. 52
Issue 11
Pg. 946-50
(Nov 2015)
ISSN: 0974-7559 [Electronic] India |
PMID | 26615341
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Adolescent
- Adult
- Epstein-Barr Virus Infections
(etiology, transmission, virology)
- Herpesvirus 4, Human
(genetics)
- Humans
- India
(epidemiology)
- Kidney Transplantation
(adverse effects)
- Liver Transplantation
(adverse effects)
- Polymerase Chain Reaction
- Transplant Recipients
(statistics & numerical data)
- Transplants
(virology)
- Young Adult
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