IMMUNODIAGNOSIS OF HUMAN STRONGYLOIDIASIS: USE OF SIX DIFFERENT ANTIGENIC FRACTIONS FROM Strongyloides venezuelensis PARASITIC FEMALES.

Abstract

The aim of this study was to evaluate six different antigenic fractions from Strongyloides venezuelensis parasitic females for the immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from S. venezuelensis parasitic females were prepared in phosphate-buffered saline (SSF and SMF, respectively), Tris-HCl (TSF and TMF, respectively), and an alkaline buffer (ASF and AMF, respectively). Serum samples obtained from patients with strongyloidiasis or, other parasitic diseases, and healthy individuals were analyzed by enzyme-linked immunosorbent assay (ELISA). Soluble fractions SSF, TSF, and ASF showed 85.0%, 75.0%, and 80.0% sensitivity and 93.1%, 93.1%, and 87.5% specificity, respectively. Membrane fractions SMF, TMF, and AMF showed 80.0%, 75.0%, and 85.0% sensitivity, and 95.8%, 90.3%, and 91.7% specificity, respectively. In conclusion, the present results suggest that the fractions obtained from parasitic females, especially the SSF and SMF, could be used as alternative antigen sources in the serodiagnosis of human strongyloidiasis.

• Authors: Marcelo Andreetta Corral, Fabiana Martins de Paula, Maiara Gottardi, Dirce Mary Correia Lima Meisel, Vera Lucia Pagliusi Castilho, Elenice Messias do Nascimento Gonçalves, Pedro Paulo Chieffi, Ronaldo Cesar Borges Gryschek
• Journal: Revista do Instituto de Medicina Tropical de Sao Paulo (Rev Inst Med Trop Sao Paulo) 2015 Sep-Oct Vol. 57 Issue 5 Pg. 427-30 ISSN: 1678-9946 [Electronic] Brazil
• PMID: 26603231 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Helminth
• Antigens, Helminth

Topics

• Animals
• Antibodies, Helminth (blood)
• Antigens, Helminth (immunology)
• Case-Control Studies
• Enzyme-Linked Immunosorbent Assay (methods)
• Female
• Humans
• Rats
• Sensitivity and Specificity
• Strongyloides (immunology)
• Strongyloidiasis (diagnosis)