Cartilage oligomeric matrix protein and matrix metalloproteinase-3 expression in the serum and joint fluid of a reversible osteoarthritis rabbit model.

Abstract

The main pathological characteristic of osteoarthritis (OA) is cartilage damage. We explored cartilage oligomeric matrix protein (COMP) and matrix metalloproteinase-3 (MMP-3) changes during articular cartilage injury and repair. Rabbits were randomly divided into the following: a blank control group; groups M1, M2, and M3, in which breaking was performed for 2, 4, and 6 weeks, respectively; and groups L1, L2, and L3, in which breaking was discontinued for 2, 4, and 6 weeks, respectively, following a 4-week recovery period. There are 7 rabbits in each group. The degree of cartilage damage in each group was scored (OA score). An enzyme-linked immunosorbent assay was used to detect COMP and MMP-3 levels in serum and joint fluid. The OA scores were 3.89 ± 2.31, 7.21 ± 2.31, and 10.88 ± 2.08 points in groups M1, M2, and M3, respectively (P < 0.05). COMP and MMP-3 levels were significantly higher in groups M1, M2, and M3 than in C. The OA score improved significantly following the 4-week recovery period (P < 0.05). COMP and MMP-3 levels began to decrease as the time following discontinuation of breaking increased, but were higher than in the control (P < 0.05). MMP- 3 and COMP levels were correlated with OA score (r > 0.7, P < 0.05). COMP and MMP-3 levels were correlated between joint fluid and serum (r = 0.899, r = 0.874, P < 0.05, respectively). Long-term joint breaking can cause articular cartilage damage. Doing some activites after the process can promote self-repair of articular cartilage. COMP and MMP-3 levels were associated with articular cartilage destruction and repair.

• Authors: X Q Chu, J J Wang, L D Dou, G Zhao
• Journal: Genetics and molecular research : GMR (Genet Mol Res) Vol. 14 Issue 4 Pg. 14207-15 (Nov 13 2015) ISSN: 1676-5680 [Electronic] Brazil
• PMID: 26600478 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Cartilage Oligomeric Matrix Protein
• Glycoproteins
• Matrix Metalloproteinase 3

Topics

• Animals
• Biomarkers (blood, metabolism)
• Cartilage Oligomeric Matrix Protein (blood, genetics, metabolism)
• Cartilage, Articular (enzymology, metabolism, pathology)
• Disease Models, Animal
• Enzyme-Linked Immunosorbent Assay
• Glycoproteins (blood, metabolism)
• Male
• Matrix Metalloproteinase 3 (biosynthesis, blood, genetics)
• Osteoarthritis, Knee (blood, genetics)
• Rabbits
• Synovial Fluid (enzymology, metabolism)
• Transcriptome