Association of CYP1A1 A4889G and T6235C polymorphisms with the risk of sporadic breast cancer in Brazilian women.

Abstract

OBJECTIVES:

We examined the influence of CYP1A1 A4889G and T6235C polymorphisms on the risk of sporadic breast cancer.

METHODS:

DNA from 742 sporadic breast cancer patients and 742 controls was analyzed using the polymerase chain reaction, followed by the restriction fragment length polymorphism technique.

RESULTS:

More patients had the CYP1A1 4889AG+GG genotype compared to controls (29.0% versus 23.2%, p=0.004). The G allele carriers had a 1.50-fold increased risk (95% CI: 1.14-1.97) of sporadic breast cancer compared to the other study participants. The frequency of the 4889AG+GG genotype among the Caucasian patients was higher than in the non-Caucasian patients (30.4% versus 20.2%, p=0.03) and controls (30.4% versus 23.2%, p=0.002). Caucasians and G allele carriers had a 1.61-fold increased risk (95% CI: 1.20-2.15) of sporadic breast cancer compared to other subjects. The CYP1A1 4889AG+GG genotype was more common among patients with a younger median age at first full-term pregnancy than among controls (33.8% versus 23.2%, p=0.001) and subjects whose first full-term pregnancies occurred at an older age (33.8% versus 26.1%, p=0.03). Women with the CYP1A1 4889AG+GG genotype and earlier first full-term pregnancies had a 1.87-fold (95% CI: 1.32-2.67) increased risk of sporadic breast cancer compared to the other study participants. Excess CYP1A1 4889AG+GG (39.8% versus27.1%, p=0.01) and 6235TC+CC (48.4% versus 35.9%, p=0.02) genotypes were also observed in patients with grade I and II tumors compared to patients with grade III tumors and controls (39.8% versus 23.2%, p=0.04; 48.4% versus 38.6%, p=0.04). The G and C allele carriers had a 2.44-fold (95% CI: 1.48-4.02) and 1.67-fold (95% CI: 1.03-2.69) increased risk, respectively, of developing grade I and II tumors compared to other subjects.

CONCLUSIONS:

The CYP1A1 A4889G and T6235C polymorphisms may alter the risk of sporadic breast cancer in Brazilian women.

• Authors: Camila Borges Martins de Oliveira, Cássio Cardoso-Filho, Leonardo Silveira Bossi, Gustavo Jacob Lourenço, Maria Salete Costa-Gurgel, Carmen Silvia Passos Lima
• Journal: Clinics (Sao Paulo, Brazil) (Clinics (Sao Paulo)) Vol. 70 Issue 10 Pg. 680-5 (Oct 2015) ISSN: 1980-5322 [Electronic] United States
• PMID: 26598080 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytochrome P-450 CYP1A1

Topics

• Adult
• Aged
• Aged, 80 and over
• Brazil
• Breast Neoplasms (genetics)
• Cytochrome P-450 CYP1A1 (genetics)
• Female
• Genetic Association Studies
• Genetic Predisposition to Disease (genetics)
• Genotype
• Humans
• Middle Aged
• Polymerase Chain Reaction
• Polymorphism, Genetic
• Polymorphism, Restriction Fragment Length
• Pregnancy
• Risk Factors
• Young Adult