Production of a bioactive sweetener steviolbioside via specific hydrolyzing ester linkage of stevioside with a β-galactosidase.

Abstract

A β-galactosidase from Kluyveromyces lactis was found to specifically catalyze hydrolysis of the glycosyl ester linkage of stevioside to yield steviolbioside, a rare sweetener that also exists in Stevia rebaudiana leaves. In a packed bed reactor, a reaction coupling separation was realized and a production yield of steviolbioside reached 90% in 6 h. The hydrolysis product steviolbioside presented higher cytoxicity on human normal cells (hepatocytes cell L02 and intestinal epithelial cell T84) than stevioside did. Comparing to the typical chemotherapy agent, 5-fluorouracil (5-FU), steviolbioside presents much lower cytotoxicity on all assayed human normal cells; it presented notable inhibition on human hepatocarcinoma cell Hep3B, human breast cancer cell MDA-MB-231 and human pancreatic cancer cell BxPC-3. The remarkable inhibition on MDA-MB-231 cells makes steviolbioside a potential remedy for human breast cancer, when steviolbioside is served as a natural sweetener.

• Authors: Jun-ming Chen, Li Ding, Xiao-chen Sui, Yong-mei Xia, Hui-da Wan, Tong Lu
• Journal: Food chemistry (Food Chem) Vol. 196 Pg. 155-60 (Apr 01 2016) ISSN: 1873-7072 [Electronic] England
• PMID: 26593477 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Diterpenes, Kaurane
• Glucosides
• Sweetening Agents
• steviolbioside
• stevioside
• beta-Galactosidase

Topics

• Diterpenes, Kaurane (chemistry)
• Glucosides (chemistry)
• Humans
• Sweetening Agents (chemistry)
• beta-Galactosidase (chemistry)