[BETA-III TUBULIN AS A POTENTIAL TARGET FOR BLOCKING INVASIVE GROWTH OF MALIGNANT EPITHELIAL TUMORS].

Abstract

Invasive growth is the first step of metastatic cascade in the growth of malignant tumors. The mobility of cells, which is a necessary factor of the invasive growth of malignant tumors, is closely linked to the dynamic structure of cytoskeleton. An important role in cell motility is played by microtubules and actin microfilaments. Microtubules consist of tubulin--a heterodimer comprising α and β subunits, which can be represented by different isotypes. The appearance of beta-III tubulin in a tumor is essential for chemoresistance and prognosis of some tumors in humans. This study focuses on determining the possibility of using beta-III tubulin as a target molecule for the suppression of invasive growth. It is established that blocking of the beta-III tubulin expression in colorectal cancer cells does not affect their viability, but reduces the cell adhesion to the extracellular matrix by 40% for HT-29 (∂ = 0.0044) and by 15% (p = 0.0436) for HCT 116) and produces a four-fold decrease in the invasive activity (p = 0.0000 and 0.0001, respectively). These facts allow considering beta-III tubulin as a target molecule in the development of antitumor drugs.

• Authors: A S Portyanko, S T Akalovich, T M Doroshenko
• Journal: Eksperimental'naia i klinicheskaia farmakologiia (Eksp Klin Farmakol) Vol. 78 Issue 8 Pg. 25-8 ( 2015) ISSN: 0869-2092 [Print] Russia (Federation)
• PMID: 26591579 (Publication Type: English Abstract, Journal Article)

Chemical References

• Neoplasm Proteins
• TUBB3 protein, human
• Tubulin

Topics

• Cell Adhesion
• Cell Line, Tumor
• Cell Movement
• Colorectal Neoplasms (metabolism, pathology)
• Gene Expression Regulation, Neoplastic
• Humans
• Neoplasm Invasiveness
• Neoplasm Proteins (antagonists & inhibitors, biosynthesis)
• Neoplasms, Glandular and Epithelial (metabolism, pathology)
• Tubulin (biosynthesis)