Abstract | BACKGROUND: Allogenic platelet transfusions (PT) are administered to treat excessive bleeding in patients on P2Y12 receptor inhibitors (RI). We assessed the effect of ex vivo and in vivo PT on platelet activation and aggregation in patients on dual antiplatelet therapy. METHODS AND RESULTS: In the Antagonize P2Y12 Treatment Inhibitors by Transfusion of Platelets in an Urgent or Delayed Timing After Acute Coronary Syndrome or Percutaneous Coronary Intervention Presentation- Acute Coronary Syndrome (APTITUDE-ACS) study, patients presenting with acute coronary syndrome or for elective percutaneous coronary intervention, receiving loading doses of clopidogrel (600 mg, n=13 or 900 mg, n=12), prasugrel 60 mg (n=10), or ticagrelor 180 mg (n=10) were included. PT was performed ex vivo by mixing platelet-rich plasma from blood sampling performed at baseline in increasing proportions with platelet-rich plasma sampled 4 hours after loading dose. The percentage restoration of residual platelet aggregation achieved with 80% proportion PT (residual platelet aggregation 80% PT mix/residual platelet aggregation baseline×100) significantly decreased with increasing potency of P2Y12 RI (83.9±11%, 73±14%, 66.3±15%, 40.9±19% for clopidogrel 600 mg, clopidogrel 900 mg, prasugrel, and ticagrelor, respectively; P for trend <0.0001). In the APTITUDE- Coronary Artery Bypass Graft (APTITUDE-CABG) study, vasodilator-stimulated phosphoprotein-platelet reactivity index, a specific marker of the P2Y12 RI drug-effect, was assessed before and after in vivo PT administered for excessive bleeding in patients undergoing cardiac surgery while on a maintenance dose of aspirin and clopidogrel (n=45), prasugrel (n=6), or ticagrelor (n=3). When compared with baseline, there was a significant relative increase of 23.1% in platelet activation after PT transfusion (42.2±23.6% versus 56.6±18.2%; P=0.0008). CONCLUSIONS: CLINICAL TRIAL REGISTRATION: URL: http://www.recherche-biomedicale.sante.gouv.fr/pro/comites/coordonnees.htm and http://www.cnil.fr/. Unique identifiers: No. 301111 and No. 1547216v0.
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Authors | Stephen A O'Connor, Julien Amour, Anne Mercadier, Réjane Martin, Mathieu Kerneis, Jérémie Abtan, Delphine Brugier, Johanne Silvain, Olivier Barthélémy, Pascal Leprince, Gilles Montalescot, Jean-Philippe Collet, ACTION Study Group |
Journal | Circulation. Cardiovascular interventions
(Circ Cardiovasc Interv)
Vol. 8
Issue 11
Pg. e002786
(Nov 2015)
ISSN: 1941-7632 [Electronic] United States |
PMID | 26553698
(Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 American Heart Association, Inc. |
Chemical References |
- Platelet Aggregation Inhibitors
- Purinergic P2Y Receptor Antagonists
- Clopidogrel
- Prasugrel Hydrochloride
- Ticagrelor
- Adenosine
- Ticlopidine
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Topics |
- Acute Coronary Syndrome
(complications, therapy)
- Adenosine
(administration & dosage, adverse effects, analogs & derivatives)
- Aged
- Clopidogrel
- Female
- Humans
- Male
- Middle Aged
- Percutaneous Coronary Intervention
- Platelet Aggregation
(drug effects)
- Platelet Aggregation Inhibitors
(administration & dosage, adverse effects)
- Platelet Function Tests
- Platelet Transfusion
- Postoperative Hemorrhage
(etiology, prevention & control)
- Prasugrel Hydrochloride
(administration & dosage, adverse effects)
- Purinergic P2Y Receptor Antagonists
(administration & dosage, adverse effects)
- Ticagrelor
- Ticlopidine
(administration & dosage, adverse effects, analogs & derivatives)
- Transplantation, Homologous
- Treatment Outcome
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