Abstract | INTRODUCTION:
Thromboxane A2 (TXA2) can induce the platelet aggregation and lead to thrombosis. This will cause the low-reflow phenomenon after ischemic stroke and aggravate the damage of brain issues. Therefore, it is potential to develop the drugs inhibiting TXA2 pathway to treat cerebral ischemia. AIM: This study aims to prove the protective effect of N2 (4-(2-(1H-imidazol-1-yl) ethoxy)-3-methoxybenzoic acid) on focal cerebral ischemia and reperfusion injury through platelet aggregation inhibition. MATERIALS AND METHODS:
Middle cerebral artery occlusion/reperfusion (MCAO/R) is used as the animal model. Neurological deficit score, Morris water maze, postural reflex test, Limb-use asymmetry test, infarct volume, and water content were performed to evaluate the protective effect of N2 in MCAO/R rats. 9, 11-dieoxy-11α, 9α-methanoepoxyprostaglandin F2α ( U46619) or adenosine diphosphate ( ADP) was used as the inducer of platelet aggregation. RESULTS AND CONCLUSIONS: N2 can improve the motor function, learning and memory ability in MCAO/R rats while reducing the infarct volume. N2 can inhibit TXA2 formation but promote PGI2, and can inhibit platelet aggregation induced by U46619 and ADP. Further, N2 inhibits thrombosis with a minor adverse effect of bleeding than Clopidogrel. In conclusion, N2 can produce the protective effect on MCAO/R brain injury through inhibiting TXA2 formation, platelet aggregation and thrombosis.
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Authors | Lijian Yang, Xi Chen, Siqi Wang, Yuxiang Fei, Ding Wang, Yunman Li, Guangwei He, Qiang Wu, Shaoxing Chu, Weirong Fang |
Journal | Thrombosis research
(Thromb Res)
Vol. 136
Issue 6
Pg. 1310-7
(Dec 2015)
ISSN: 1879-2472 [Electronic] United States |
PMID | 26553017
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ltd. All rights reserved. |
Chemical References |
- 4-(2-(1H-imidazol-1-yl)ethoxy)-3-methoxybenzoic acid
- Imidazoles
- dazoxiben
- Thromboxane A2
- Adenosine Diphosphate
- 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
- Epoprostenol
- Vanillic Acid
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Topics |
- 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
(chemistry)
- Adenosine Diphosphate
(chemistry)
- Animals
- Arteriovenous Shunt, Surgical
- Blood Coagulation
- Brain
(pathology)
- Brain Ischemia
(drug therapy)
- Edema
(pathology)
- Enzyme-Linked Immunosorbent Assay
- Epoprostenol
(chemistry)
- Female
- Imidazoles
(chemistry)
- Male
- Maze Learning
- Platelet Aggregation
(drug effects)
- Rats
- Rats, Sprague-Dawley
- Stroke
(drug therapy)
- Thrombosis
(physiopathology)
- Thromboxane A2
(chemistry)
- Vanillic Acid
(analogs & derivatives, chemistry)
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