Abstract |
Among the eight phytochemicals ( dihydrocarveol, sinapic acid, vanillic acid, ethylgallate, myrtenol, transcarveol, p-methoxycinnamic acid, and isoferulic acid) we tested, p-methoxycinnamic acid (p-MCA) [10 μM] showed the most potent in vitro growth inhibition on human colon adenocarcinoma (HCT-116 cells). Antiproliferative activity of p-MCA at 24h was associated with DNA damage, morphological changes and the results were comparable with doxorubicin. p-MCA induced phosphatidylserine translocation, increased the levels of reactive oxygen species (ROS), thiobarbituric acid reactive substances ( TBARS), protein carbonyl content (PCC) and decreased enzymic antioxidant status (SOD, CAT, GPx) in HCT-116. p-MCA treatment increased the percentage of apoptotic cells, decreased the mitochondrial membrane potential and triggered cytochrome C release to cytosol. The induction of apoptosis by p-MCA was accompanied by an increase in caspase 3 and caspase 9 activities, increased expression of Bax and decreased expression of Bcl-2. Thus p-MCA induces mitochondria mediated intrinsic pathway of apoptosis in HCT-116 and has potential for treatment and prevention of colon cancer.
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Authors | Sivagami Gunasekaran, Karthikkumar Venkatachalam, Nalini Namasivayam |
Journal | Environmental toxicology and pharmacology
(Environ Toxicol Pharmacol)
Vol. 40
Issue 3
Pg. 966-74
(Nov 2015)
ISSN: 1872-7077 [Electronic] Netherlands |
PMID | 26546748
(Publication Type: Journal Article)
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Copyright | Copyright © 2015 Elsevier B.V. All rights reserved. |
Chemical References |
- Antineoplastic Agents
- Cinnamates
- Phytochemicals
- Reactive Oxygen Species
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Topics |
- Adenocarcinoma
(metabolism)
- Antineoplastic Agents
(pharmacology)
- Apoptosis
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Cinnamates
(pharmacology)
- Colonic Neoplasms
(metabolism)
- HCT116 Cells
- HT29 Cells
- Humans
- Membrane Potential, Mitochondrial
(drug effects)
- Mitochondria
(drug effects)
- Oxidative Stress
(drug effects)
- Phytochemicals
(pharmacology)
- Reactive Oxygen Species
(metabolism)
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