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p-Methoxycinnamic acid, an active phenylpropanoid induces mitochondrial mediated apoptosis in HCT-116 human colon adenocarcinoma cell line.

Abstract
Among the eight phytochemicals (dihydrocarveol, sinapic acid, vanillic acid, ethylgallate, myrtenol, transcarveol, p-methoxycinnamic acid, and isoferulic acid) we tested, p-methoxycinnamic acid (p-MCA) [10 μM] showed the most potent in vitro growth inhibition on human colon adenocarcinoma (HCT-116 cells). Antiproliferative activity of p-MCA at 24h was associated with DNA damage, morphological changes and the results were comparable with doxorubicin. p-MCA induced phosphatidylserine translocation, increased the levels of reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), protein carbonyl content (PCC) and decreased enzymic antioxidant status (SOD, CAT, GPx) in HCT-116. p-MCA treatment increased the percentage of apoptotic cells, decreased the mitochondrial membrane potential and triggered cytochrome C release to cytosol. The induction of apoptosis by p-MCA was accompanied by an increase in caspase 3 and caspase 9 activities, increased expression of Bax and decreased expression of Bcl-2. Thus p-MCA induces mitochondria mediated intrinsic pathway of apoptosis in HCT-116 and has potential for treatment and prevention of colon cancer.
AuthorsSivagami Gunasekaran, Karthikkumar Venkatachalam, Nalini Namasivayam
JournalEnvironmental toxicology and pharmacology (Environ Toxicol Pharmacol) Vol. 40 Issue 3 Pg. 966-74 (Nov 2015) ISSN: 1872-7077 [Electronic] Netherlands
PMID26546748 (Publication Type: Journal Article)
CopyrightCopyright © 2015 Elsevier B.V. All rights reserved.
Chemical References
  • Antineoplastic Agents
  • Cinnamates
  • Phytochemicals
  • Reactive Oxygen Species
Topics
  • Adenocarcinoma (metabolism)
  • Antineoplastic Agents (pharmacology)
  • Apoptosis
  • Cell Line, Tumor
  • Cell Proliferation (drug effects)
  • Cell Survival (drug effects)
  • Cinnamates (pharmacology)
  • Colonic Neoplasms (metabolism)
  • HCT116 Cells
  • HT29 Cells
  • Humans
  • Membrane Potential, Mitochondrial (drug effects)
  • Mitochondria (drug effects)
  • Oxidative Stress (drug effects)
  • Phytochemicals (pharmacology)
  • Reactive Oxygen Species (metabolism)

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