Abstract | BACKGROUND:
Phospholipases A2 (PLA2s) are abundant components of snake venoms that have been extensively studied due to their pharmacological and pathophysiological effects on living organisms. This study aimed to assess the antitumor potential of BthTX-I, a basic myotoxic PLA2 isolated from Bothrops jararacussu venom, by evaluating in vitro processes of cytotoxicity, modulation of the cell cycle and induction of apoptosis in human (HL-60 and HepG2) and murine (PC-12 and B16F10) tumor cell lines. METHODS: RESULTS: It was observed that BthTX-I was cytotoxic to all evaluated tumor cell lines, reducing their viability in 40 to 50 %. The myotoxin showed modulating effects on the cell cycle of PC-12 and B16F10 cells, promoting delay in the G0/G1 phase. Additionally, flow cytometry analysis indicated cell death mainly by apoptosis. B16F10 was more susceptible to the effects of BthTX-I, with ~40 % of the cells analyzed in apoptosis, followed by HepG2 (~35 %), PC-12 (~25 %) and HL-60 (~4 %). CONCLUSIONS: These results suggest that BthTX-I presents antitumor properties that may be useful for developing new therapeutic strategies against cancer.
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Authors | Cássio Prinholato da Silva, Tássia R Costa, Raquel M Alves Paiva, Adélia C O Cintra, Danilo L Menaldo, Lusânia M Greggi Antunes, Suely V Sampaio |
Journal | The journal of venomous animals and toxins including tropical diseases
(J Venom Anim Toxins Incl Trop Dis)
Vol. 21
Pg. 44
( 2015)
ISSN: 1678-9199 [Print] Brazil |
PMID | 26539212
(Publication Type: Journal Article)
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