Dual subcellular compartment delivery of doxorubicin to overcome drug resistant and enhance antitumor activity.

Abstract

In order to overcome drug resistant and enhance antitumor activity of DOX, a new pH-sensitive micelle (DOX/DQA-DOX@DSPE-hyd-PEG-AA) was prepared to simultaneously deliver DOX to nucleus and mitochondria. Drug released from DOX/DQA-DOX@DSPE-hyd-PEG-AA showed a pH-dependent manner. DOX/DQA-DOX@DSPE-hyd-PEG-AA induced the depolarization of mitochondria and apoptosis in MDA-MB-231/ADR cells and A549 cells, which resulted in the high cytotoxicity of DOX/DQA-DOX@DSPE-hyd-PEG-AA against MDA-MB-231/ADR cells and A549 cells. Confocal microscopy confirmed that DOX/DQA-DOX@DSPE-hyd-PEG-AA simultaneously delivered DQA-DOX and DOX to the mitochondria and nucleus of tumor cell. After DOX/DQA-DOX@DSPE-hyd-PEG-AA was injected to the tumor-bearing nude mice by the tail vein, DOX was mainly found in tumor tissue. But DOX was widely distributed in the whole body after the administration of free DOX. Compared with free DOX, the same dose of DOX/DQA-DOX@DSPE-hyd-PEG-AA significantly inhibited the growth of DOX-resistant tumor in tumor-bearing mice without obvious systemic toxicity. Therefore, dual subcellular compartment delivery of DOX greatly enhanced the antitumor activity of DOX on DOX-resistant tumor. DOX/DQA-DOX@DSPE-hyd-PEG-AA has the potential in target therapy for DOX-resistant tumor.

• Authors: Yan-feng Song, Dao-zhou Liu, Ying Cheng, Miao Liu, Wei-liang Ye, Bang-le Zhang, Xin-you Liu, Si-yuan Zhou
• Journal: Scientific reports (Sci Rep) Vol. 5 Pg. 16125 (Nov 04 2015) ISSN: 2045-2322 [Electronic] England
• PMID: 26530454 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibiotics, Antineoplastic
• Drug Carriers
• Micelles
• Phosphatidylethanolamines
• polyethylene glycol-distearoylphosphatidylethanolamine
• Polyethylene Glycols
• Doxorubicin
• Caspase 3

Topics

• Animals
• Antibiotics, Antineoplastic (metabolism, pharmacology, therapeutic use, toxicity)
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Doxorubicin (metabolism, pharmacology, therapeutic use)
• Drug Carriers (chemistry)
• Female
• Humans
• Hydrogen-Ion Concentration
• Membrane Potential, Mitochondrial (drug effects)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Micelles
• Neoplasms (drug therapy, pathology)
• Phosphatidylethanolamines (chemistry)
• Polyethylene Glycols (chemistry)
• Tissue Distribution
• Transplantation, Heterologous