Abstract | BACKGROUND/AIMS: METHODS: We developed a model of peritoneal fibrosis by intraperitoneally injecting 4.25%- glucose PD fluids and lipopolysaccharide to Sprague-Dawley rats. The rats received daily intraperitoneal injections of NaHS (56 μg/kg), an H2S donor. After 28 days, the peritoneal equilibration test (PET) was used to assess peritoneal function. At the end of dialysis, the rats were killed and parietal peritoneum was harvested for microscopic examination and immunohistochemistry. RESULTS: On the 28th day, the parietal peritoneum of the PD rats markedly thickened as a result of increased depositions of type III collagen and fibronectin. Moreover, the number of ED-1-positive cells and the expressions of monocyte chemoattractant protein-1, transforming growth factor-β1 (TGF-β1), α-smooth muscle actin and CD31 were significantly increased in the fibrotic peritoneum. Administration of NaHS markedly decreased the biomarkers of inflammation, fibrosis and angiogenesis in the peritoneum. NaHS also improved peritoneal function assessed by PET. CONCLUSION: Exogenous H2S ameliorates the pathologic process of peritonitis via attenuating inflammatory events and TGF-β1 synthesis. These results suggest that H2S may be a potential therapy against peritoneal fibrosis during chronic PD. In the future, compounds releasing H2S at controlled rate will be assessed as potential candidates to treat peritoneal fibrosis.
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Authors | Ying Lu, Luyan Gao, Lingyun Li, Ye Zhu, Zhi Wang, Huaying Shen, Kai Song |
Journal | Nephron
(Nephron)
Vol. 131
Issue 3
Pg. 210-9
( 2015)
ISSN: 2235-3186 [Electronic] Switzerland |
PMID | 26529331
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2015 S. Karger AG, Basel. |
Chemical References |
- Actins
- Ccl2 protein, rat
- Chemokine CCL2
- Collagen Type III
- Fibronectins
- Lipopolysaccharides
- Transforming Growth Factor beta1
- smooth muscle actin, rat
- Hydrogen Sulfide
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Topics |
- Actins
(biosynthesis, genetics)
- Animals
- Chemokine CCL2
(biosynthesis)
- Collagen Type III
(metabolism)
- Dialysis
- Fibronectins
(metabolism)
- Hydrogen Sulfide
(therapeutic use)
- Inflammation
(drug therapy, pathology)
- Injections, Intraperitoneal
- Lipopolysaccharides
(pharmacology)
- Male
- Peritoneal Fibrosis
(drug therapy, pathology)
- Peritonitis
(drug therapy, pathology)
- Rats
- Rats, Sprague-Dawley
- Transforming Growth Factor beta1
(antagonists & inhibitors, biosynthesis)
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