Hyperin attenuates inflammation by activating PPAR-γ in mice with acute liver injury (ALI) and LPS-induced RAW264.7 cells.

Abstract

Hyperin (HP) is a flavonoid compound found in various plants like Ericaceae, Guttifera and Celastraceae. The present study has revealed that HP has a variety of pharmacological effects including anti-oxidant, anticancer, and anti-coagulant, especially anti-inflammatory. However, the potential molecular mechanism of anti-inflammatory is still unrevealed. In this study, HP not only significantly attenuated inflammation in C57BL/6J mice with acute liver injury (ALI), but also reduced the expression of TNF-α and IL-6 in lipopolysaccharide (LPS)-induced RAW264.7 cells. Furthermore, our findings showed that HP remarkably induced the expression of PPAR-γ in vivo and in vitro. Interestingly, compared with the HP treatment group, a specific blocking agent of PPAR-γ T0070907 and PPAR-γ small interfering (si)-RNA-mediated silencing in RAW264.7 cells were used to evaluate the involvement of HP in alleviating LPS-induced inflammation. More importantly, over-expression of PPAR-γ had an opposite effect on the expression of TNF-α and IL-6 in LPS-induced RAW264.7 cells after treatment with HP. In addition, HP remarkably inhibited the expression of P-ERK1/2 and P-P38 MAPK. Taken together, all the above results indicate that HP may serve as an effective modulator of PPAR-γ, further down-regulating ERK1/2 and p38 MAPK during the pathogenesis of inflammation.

• Authors: Cheng Huang, Yang Yang, Wan-Xia Li, Xiao-Qin Wu, Xiao-Feng Li, Tao-Tao Ma, Lei Zhang, Xiao-Ming Meng, Jun Li
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 29 Issue 2 Pg. 440-447 (Dec 2015) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 26526086 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Interleukin-6
• Lipopolysaccharides
• PPAR gamma
• Tumor Necrosis Factor-alpha
• interleukin-6, mouse
• hyperoside
• Quercetin
• p38 Mitogen-Activated Protein Kinases

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Chemical and Drug Induced Liver Injury (drug therapy, pathology)
• Interleukin-6 (antagonists & inhibitors)
• Lipopolysaccharides (pharmacology)
• MAP Kinase Signaling System (drug effects)
• Macrophages (drug effects, metabolism)
• Mice
• Mice, Inbred C57BL
• PPAR gamma (agonists, antagonists & inhibitors)
• Quercetin (analogs & derivatives, pharmacology)
• RAW 264.7 Cells
• Tumor Necrosis Factor-alpha (antagonists & inhibitors)
• p38 Mitogen-Activated Protein Kinases (antagonists & inhibitors)