Panax Notoginseng Saponins Ameliorates Coxsackievirus B3-Induced Myocarditis by Activating the Cystathionine-γ-Lyase/Hydrogen Sulfide Pathway.

Abstract

This study is to determine the therapeutic effects of Panax notoginseng saponins (PNSs) on coxsackievirus B3 (CVB3)-induced myocarditis, and whether cystathionine-γ-lyase (CSE)/hydrogen sulfide (H2S) pathway is involved. Mouse model of myocarditis was induced by CVB3 infection, and the mice were subjected to vehicle (saline) or drug treatments (sodium bisulfide (NaHS), propargylglycine (PAG), or PNSs). The results showed that there were inflammatory cell infiltrations, interstitial edemas, and elevated inflammatory cytokines, in CVB3-induced myocarditis. PAG administration increased, whereas NaHS treatment decreased the severity of the myocarditis. PNS treatment dramatically alleviated these myocardial injuries and decreased the viral messenger RNA (mRNA) expression by the enhanced expression of CSE/H2S pathway. Moreover, the therapeutic effects of PNSs on myocarditis were stronger than those of NaHS. Finally, the effect of PNSs on CSE/H2S pathway and cardiac cell protection were verified in cultured cardiac cells. PNSs may be a promising medication for viral myocarditis therapy.

• Authors: Lulu Pan, Yuanhai Zhang, Jiacheng Lu, Zhimin Geng, Lianhong Jia, Xing Rong, Zhenquan Wang, Qifeng Zhao, Rongzhou Wu, Maoping Chu, Chunxiang Zhang
• Journal: Journal of cardiovascular translational research (J Cardiovasc Transl Res) Vol. 8 Issue 9 Pg. 536-44 (Dec 2015) ISSN: 1937-5395 [Electronic] United States
• PMID: 26525047 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Plant Preparations
• RNA, Small Interfering
• Saponins
• Cystathionine gamma-Lyase
• Hydrogen Sulfide

Topics

• Analysis of Variance
• Animals
• Biomarkers (metabolism)
• Blotting, Western
• Coxsackievirus Infections (drug therapy, physiopathology)
• Cystathionine gamma-Lyase (metabolism)
• Disease Models, Animal
• Enterovirus B, Human (drug effects, isolation & purification)
• Enzyme-Linked Immunosorbent Assay
• Humans
• Hydrogen Sulfide (metabolism)
• Male
• Mice
• Mice, Inbred BALB C
• Myocarditis (drug therapy, virology)
• Panax notoginseng
• Phytotherapy (methods)
• Plant Preparations (administration & dosage)
• RNA, Small Interfering (metabolism)
• Random Allocation
• Real-Time Polymerase Chain Reaction (methods)
• Saponins (therapeutic use)
• Signal Transduction