[Effect of Alantolactone on Proliferation of RPMI-8226 Cells and Its Possible Mechnism].

Abstract

OBJECTIVE:

To investigate the effect of alantolactone on perliferation and apoptosis of multiple myeloma (MM) RPMI-8226 cells, and to explore its possible mechism in vitro and in vivo.

METHODS:

The RPMI-8226 cells were treated with alantolactone (1, 2.5, 5, 7.5 and 10 µmol/L) for 48 h, cell viability was detected by CCK-8 assay and the value of IC50 was calculated; The RPMI-8226 cells were treated with alantolactone (2.5, 5 and 7.5 µmol/L) for 48 h, the apoptotic rate was detected by flow cytmetry with Annexin V/PI staining; the expression level of cleaved caspase-3 and phosphorylation of ERK were measured by Western blot; the nude mice was used to further confirm the proapoptotic effect of alantolactone on MM cells in vivo.

RESULTS:

The alantolactone inhibited RPMI-8226 cell viability remarkably with a dose-dependent manner; the IC50 value of RPMI-8226 cells at 48 h was 4.32 ± 0.15 µmol/L; the apoptotic rate increased observably with a dose-dependent manner; the levels of cleaved-caspase-3 increased and the phosphorylation of ERK decreased significantly; as compared to control, the volum of tumor was much smaller, the expression levels of Ki67 and p-ERK decreased.

CONCLUSION:

The alantolactone can efficiently inhibit the proliferation and induce the apoptosis of multiple myeloma RPMI-8226 cells in vitro and in vivo through inhibiting the activation of ERK signal pathway.

• Authors: Yao Yao, Yue-Yue Sun, Dan-Dan Xia, Ming-Shan Niu, Kai Zhao, Zhen-Yu Li, Ling-Yu Zeng, Kai-Lin Xu
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 23 Issue 5 Pg. 1336-40 (Oct 2015) ISSN: 1009-2137 [Print] China
• PMID: 26524033 (Publication Type: Journal Article)

Chemical References

• Lactones
• Sesquiterpenes, Eudesmane
• CASP3 protein, human
• Caspase 3
• alantolactone

Topics

• Animals
• Apoptosis
• Caspase 3 (metabolism)
• Cell Line, Tumor (drug effects)
• Cell Proliferation (drug effects)
• Humans
• Lactones (pharmacology)
• Mice
• Mice, Nude
• Multiple Myeloma (pathology)
• Sesquiterpenes, Eudesmane (pharmacology)
• Signal Transduction