Abstract | OBJECTIVE: To explore the effects of polydatin on human kidney tubular epithelial cells (HK-2 cells) with oxygen- glucose deprivation/re-oxygenation (OGD/R)-induced injury and potential mechanisms. METHODS: HK-2 cells were cultured under normal or OGD/R condition with different drug treatment methods, including 10, 20 and 40 μmol/L polydatin, and 1 μmol/L Wortmannin, a specific phosphatidylinositol 3-kinase (PI3K) inhibitor. MTT assay was used to detect the survival ability of cells in different groups. The contents of tumor necrosis factor α (TNF-α) and interleukine 1β (IL-1β) in supernatant fluids of the cultured cells were examined by ELISA. And Western blotting was performed to determine the protein levels of total Akt (t-Akt), phospho-Akt (p-Akt) and Sonic hedgehog (Shh) in different groups. RESULTS:
Polydatin significantly improved the viability of cells with OGD/R treatment, and apparently inhibited the secretion of TNF-α and IL-1β induced by OGD/R. The inhibition of PI3K/Akt signaling pathway counteracted the anti- inflammation and pro-survival effects of polydatin and blocked the protein expression of Shh in HK-2 cells. The exogenous addition of human recombinant Shh protein not only improved the survival of cells with OGD/R treatment, but also inhibited the inflammation induced by OGD/R in HK-2 cells. CONCLUSION:
Polydatin can exert protective effects on HK-2 cells with OGD/R through regulating the PI3K/Akt-dependent Shh pathway.
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Authors | Qiuhong Meng, Hongbao Liu, Jianbo Wang |
Journal | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
(Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi)
Vol. 31
Issue 11
Pg. 1452-7
(Nov 2015)
ISSN: 1007-8738 [Print] China |
PMID | 26522350
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Glucosides
- Hedgehog Proteins
- SHH protein, human
- Stilbenes
- Phosphatidylinositol 3-Kinases
- Proto-Oncogene Proteins c-akt
- polydatin
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Topics |
- Cell Survival
(drug effects)
- Cells, Cultured
- Cytoprotection
- Glucosides
(pharmacology)
- Hedgehog Proteins
(physiology)
- Humans
- Hypoxia
(pathology)
- Phosphatidylinositol 3-Kinases
(physiology)
- Proto-Oncogene Proteins c-akt
(physiology)
- Signal Transduction
(physiology)
- Stilbenes
(pharmacology)
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