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Gypensapogenin H, a novel dammarane-type triterpene induces cell cycle arrest and apoptosis on prostate cancer cells.

Abstract
Gypensapogenin H (GH) is a novel dammarane-type triterpenes obtained from hydrolyzate of total saponins from Gynostemma pentaphyllum and its anti-tumor activity has been studied in previous work. In this study, we report the effects of this compound on human prostate cancer cells (DU145 and 22RV-1). It significantly inhibited proliferation, decreased survival, led to G1 cell cycle arrest and induced apoptosis in both cell lines, while having lesser effect on the growth of normal human gastric mucosa cells (GES-1), embryonic kidney cells (HEK293) and lung fibroblast cells (MRC5). Consistent with these phenotypes, we observed decreased expression of the cell cycle-related proteins cyclinD1, and CDK4, and increased expression of p21 in GH-treated cells. Besides, the anti-apoptotic Bcl-2 protein decreased in a dose-dependent manner, while Bax, cleaved caspase-3 and -9 increased upon GH treatment. Taken together, these results indicated GH exerted promising anticancer activity, and may represent a potential agent for the treatment of prostate cancer.
AuthorsXiao-Shu Zhang, Chen Zhao, Wei-zhuo Tang, Xiao-jun Wu, Yu-Qing Zhao
JournalSteroids (Steroids) Vol. 104 Pg. 276-83 (Dec 2015) ISSN: 1878-5867 [Electronic] United States
PMID26514740 (Publication Type: Journal Article)
CopyrightCopyright © 2015. Published by Elsevier Inc.
Chemical References
  • Antineoplastic Agents, Phytogenic
  • Saponins
  • Triterpenes
  • gypensapogenin H
  • dammarane
Topics
  • Antineoplastic Agents, Phytogenic (chemistry, isolation & purification, pharmacology)
  • Apoptosis (drug effects)
  • Cell Cycle Checkpoints (drug effects)
  • Cell Line
  • Cell Proliferation (drug effects)
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor
  • Gynostemma (chemistry)
  • HEK293 Cells
  • Humans
  • Male
  • Molecular Conformation
  • Prostatic Neoplasms (drug therapy, pathology)
  • Saponins (chemistry, isolation & purification, pharmacology)
  • Structure-Activity Relationship
  • Triterpenes (chemistry, isolation & purification, pharmacology)

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