Abstract |
Melatonin is an important secondary messenger in plant innate immunity against the bacterial pathogen Pseudomonas syringe pv. tomato (Pst) DC3000 in the salicylic acid (SA)- and nitric oxide (NO)-dependent pathway. However, the metabolic homeostasis in melatonin-mediated innate immunity is unknown. In this study, comparative metabolomic analysis found that the endogenous levels of both soluble sugars ( fructose, glucose, melibose, sucrose, maltose, galatose, tagatofuranose and turanose) and glycerol were commonly increased after both melatonin treatment and Pst DC3000 infection in Arabidopsis. Further studies showed that exogenous pre-treatment with fructose, glucose, sucrose, or glycerol increased innate immunity against Pst DC3000 infection in wild type (Col-0) Arabidopsis plants, but largely alleviated their effects on the innate immunity in SA-deficient NahG plants and NO-deficient mutants. This indicated that SA and NO are also essential for sugars and glycerol-mediated disease resistance. Moreover, exogenous fructose, glucose, sucrose and glycerol pre-treatments remarkably increased endogenous NO level, but had no significant effect on the endogenous melatonin level. Taken together, this study highlights the involvement of sugars and glycerol in melatonin-mediated innate immunity against bacterial pathogen in SA and NO-dependent pathway in Arabidopsis.
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Authors | Yongqiang Qian, Dun-Xian Tan, Russel J Reiter, Haitao Shi |
Journal | Scientific reports
(Sci Rep)
Vol. 5
Pg. 15815
(Oct 28 2015)
ISSN: 2045-2322 [Electronic] England |
PMID | 26508076
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Arabidopsis Proteins
- Melatonin
- Salicylic Acid
- Glycerol
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Topics |
- Arabidopsis
(immunology, metabolism, microbiology)
- Arabidopsis Proteins
(immunology, metabolism)
- Disease Resistance
(immunology)
- Gene Expression Regulation, Plant
(immunology)
- Glycerol
(immunology, metabolism)
- Melatonin
(immunology, metabolism)
- Metabolomics
(methods)
- Plant Diseases
(immunology, microbiology)
- Plant Immunity
(immunology)
- Plants, Genetically Modified
(immunology, microbiology)
- Salicylic Acid
(immunology, metabolism)
- Signal Transduction
(immunology)
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