Impact of OAS1 Exon 7 rs10774671 Genetic Variation on Liver Fibrosis Progression in Egyptian HCV Genotype 4 Patients.

Abstract

The aim of this study was to assess the impact of genetic variants of oligoadenylate synthetase 1 (OAS1) single-nucleotide polymorphism (SNP) rs10774671 at the exon 7 splice acceptor site on liver fibrosis progression and hepatitis C virus (HCV) outcome in Egyptian HCV genotype 4 patients. In this study, 195 subjects were enrolled; 60 controls and 135 chronic HCV genotype 4 patients with different fibrosis grades. All subjects were genotyped for OAS1 SNP rs10774671 polymorphism by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. There was an increasing trend of liver fibrosis progression as 52.9% GG, 73.6% GA, and 83.3% AA genotypes were detected in late fibrosis patients (p = 0.025). The AA genotype was higher in the late fibrosis group than in the early fibrosis group (83.3% vs. 16.7%) (p = 0.001). The A allele was significantly affecting the liver fibrosis progression rate, more than the G allele (p = 0.001). The multivariate analysis showed that the OAS1 GA and AA genotypes were independent factors associated with liver progression (p = 0.009, odds ratio [OR] 3.467, 95% confidence interval [CI] 1.273-7.584). In addition, the A allele was associated with liver fibrosis progression (p = 0.014, OR 2.525, 95% CI 1.157-4.545). The polymorphism at OAS1 exon 7 rs3741981 might be a potential genetic marker and can be useful in the assessment of liver fibrosis progression and disease outcome in HCV-infected patients.

• Authors: Noha G Bader El Din, Mohamed A Anany, Reham M Dawood, Marwa K Ibrahim, Reem El-Shenawy, Yasmin S El Abd, Mostafa K El Awady
• Journal: Viral immunology (Viral Immunol) Vol. 28 Issue 9 Pg. 509-16 (Nov 2015) ISSN: 1557-8976 [Electronic] United States
• PMID: 26505957 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• OAS1 protein, human
• 2',5'-Oligoadenylate Synthetase

Topics

• 2',5'-Oligoadenylate Synthetase (genetics)
• Adult
• Aged
• Alleles
• Disease Progression
• Egypt
• Exons
• Female
• Genotype
• Genotyping Techniques
• Hepacivirus (classification, genetics, isolation & purification)
• Hepatitis C, Chronic (complications, virology)
• Humans
• Liver Cirrhosis (diagnosis, genetics)
• Male
• Middle Aged
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Polymorphism, Single Nucleotide
• Prognosis