Complementation and analysis of an NP mutant of influenza virus.

Abstract

Cell lines were constructed so as to express the influenza A virus nucleoprotein (NP) at levels approximating 5% of the total NP made throughout virus infection. Two types of cell lines were analyzed. One cell line (NP-5) expresses only the NP while another cell line was constructed which expresses the three viral polymerase proteins in addition to the NP (3PNP-4). Both cell lines were able to complement the growth of an NP mutant, ts56, at the non-permissive temperature. The 3PNP-4 cell line, constructed by transfecting a cell line already expressing the three polymerase proteins, continued to be able to complement viral PB2 mutants. In addition, sequence analysis was performed on the NP gene segment of A/WSN/33 and ts56 viruses. This analysis revealed that the mutant phenotype exhibited by ts56 at non-permissive temperature is due to a single serine to asparagine change (at codon 332) within the protein.

• Authors: R A Li, P Palese, M Krystal
• Journal: Virus research (Virus Res) Vol. 12 Issue 2 Pg. 97-111 (Feb 1989) ISSN: 0168-1702 [Print] Netherlands
• PMID: 2650474 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA, Viral
• NP protein, Influenza A virus
• Nucleocapsid Proteins
• Nucleoproteins
• RNA-Binding Proteins
• Viral Core Proteins
• Viral Proteins

Topics

• Amino Acid Sequence
• Base Sequence
• Cell Line, Transformed
• Cloning, Molecular
• DNA, Viral (genetics)
• Electrophoresis, Polyacrylamide Gel
• Fluorescent Antibody Technique
• Gene Expression Regulation
• Genetic Complementation Test
• Immunoblotting
• Influenza A virus (genetics)
• Molecular Sequence Data
• Mutation
• Nucleocapsid Proteins
• Nucleoproteins (biosynthesis, genetics)
• Phenotype
• Plasmids
• RNA-Binding Proteins
• Transfection
• Viral Core Proteins
• Viral Proteins (biosynthesis, genetics)