Complementation and analysis of an NP mutant of influenza virus.

Cell lines were constructed so as to express the influenza A virus nucleoprotein (NP) at levels approximating 5% of the total NP made throughout virus infection. Two types of cell lines were analyzed. One cell line (NP-5) expresses only the NP while another cell line was constructed which expresses the three viral polymerase proteins in addition to the NP (3PNP-4). Both cell lines were able to complement the growth of an NP mutant, ts56, at the non-permissive temperature. The 3PNP-4 cell line, constructed by transfecting a cell line already expressing the three polymerase proteins, continued to be able to complement viral PB2 mutants. In addition, sequence analysis was performed on the NP gene segment of A/WSN/33 and ts56 viruses. This analysis revealed that the mutant phenotype exhibited by ts56 at non-permissive temperature is due to a single serine to asparagine change (at codon 332) within the protein.
AuthorsR A Li, P Palese, M Krystal
JournalVirus research (Virus Res) Vol. 12 Issue 2 Pg. 97-111 (Feb 1989) ISSN: 0168-1702 [Print] NETHERLANDS
PMID2650474 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • DNA, Viral
  • NP protein, Influenza A virus
  • Nucleoproteins
  • RNA-Binding Proteins
  • Viral Core Proteins
  • Viral Proteins
  • Amino Acid Sequence
  • Base Sequence
  • Cell Line, Transformed
  • Cloning, Molecular
  • DNA, Viral (genetics)
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique
  • Gene Expression Regulation
  • Genetic Complementation Test
  • Immunoblotting
  • Influenza A virus (genetics)
  • Molecular Sequence Data
  • Mutation
  • Nucleoproteins (biosynthesis, genetics)
  • Phenotype
  • Plasmids
  • RNA-Binding Proteins
  • Transfection
  • Viral Core Proteins
  • Viral Proteins (biosynthesis, genetics)

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