Combination of glycosphingosomes and liposomal doxorubicin shows increased activity against dimethyl-α-benzanthracene-induced fibrosarcoma in mice.

Abstract

The present study aimed to assess the antitumor effect of glycosphingolipid-incorporated liposomes (glycosphingosomes) in combination with liposomal doxorubicin (Lip-Dox) in a mouse model of fibrosarcoma. Glycosphingosomes were prepared by incorporating glycosphingolipids isolated from Sphingomonas paucimobilis into the liposomes of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, cholesterol, and cardiolipin. Tumors were induced by administering dimethyl-α-benzanthracene, and tumor-bearing mice were treated with various formulations of Dox, including free Dox, Lip-Dox, or glycosphingosomes + Lip-Dox. Mice were observed for 90 days to monitor their survival and tumor size. Free Dox, but not Lip-Dox or a combination of glycosphingosomes and Lip-Dox, caused the substantial depletion of leukocytes and significantly increased the levels of lactate dehydrogenase and creatinine kinase in mice. Tumor-bearing mice treated with a combination of glycosphingosomes and Lip-Dox showed restricted tumor growth and increased survival when compared to those treated with free Dox or Lip-Dox. The results of the present study suggest that a combination of glycosphingosomes and Lip-Dox may prove to be very effective in the treatment of tumors.

• Authors: Masood A Khan, Ahmed N Aljarbou, Yousef H Aldebasi, Mohammed S Alorainy, Arif Khan
• Journal: International journal of nanomedicine (Int J Nanomedicine) Vol. 10 Pg. 6331-8 ( 2015) ISSN: 1178-2013 [Electronic] New Zealand
• PMID: 26504383 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• liposomal doxorubicin
• 1,2-Dipalmitoylphosphatidylcholine
• colfosceril palmitate
• Polyethylene Glycols
• 9,10-Dimethyl-1,2-benzanthracene
• Doxorubicin

Topics

• 1,2-Dipalmitoylphosphatidylcholine (analogs & derivatives, chemistry)
• 9,10-Dimethyl-1,2-benzanthracene (toxicity)
• Animals
• Cell Proliferation (drug effects)
• Chemistry, Pharmaceutical
• Doxorubicin (analogs & derivatives, chemistry, pharmacology, therapeutic use)
• Female
• Fibrosarcoma (chemically induced, drug therapy, pathology)
• Mice
• Polyethylene Glycols (chemistry, pharmacology, therapeutic use)
• Survival Analysis