Abstract | AIM: We aimed to develop a plasma protein signature for breast cancer diagnosis by using multiple reaction monitoring (MRM)-based mass spectrometry. MATERIALS AND METHODS: Based on our previous studies, we selected 124 proteins for MRM. Plasma samples from 80 patients with breast cancer and 80 healthy women were used to develop a plasma proteomic signature by an MRM approach. The proteomic signature was then validated in plasma samples from 100 patients with breast cancer and 100 healthy women. RESULTS: A total of 56 proteins were optimized for MRM. In the verification cohort, 11 proteins exhibited significantly differential expression in plasma from patients with breast cancer. Three proteins ( neural cell adhesion molecule L1-like protein, apolipoprotein C-1 and carbonic anhydrase-1) with highest statistical significance which gave consistent results for patients of stage I and II breast cancer were selected and a 3-protein signature was developed using binary logistic regression analysis [area under the curve (AUC)=0.851, sensitivity=80.6%]. The 3-protein signature showed similar performance in an independent validation cohort with an AUC of 0.797 and sensitivity of 77.2% for detection of stage I and II breast cancer. CONCLUSION: We developed a distinct plasma protein signature for breast cancer diagnosis based on an MRM-based approach, and the clinical value of the 3-protein signature was validated in an independent cohort.
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Authors | Han-Byoel Lee, Un-Beom Kang, Hyeong-Gon Moon, Jiwoo Lee, Kyung-Min Lee, Minju Yi, Yong Sun Park, Jong Won Lee, Jong-Han Yu, Seung Ho Choi, Sang Heon Cho, Cheolju Lee, Wonshik Han, Dong-Young Noh |
Journal | Anticancer research
(Anticancer Res)
Vol. 35
Issue 11
Pg. 6271-9
(Nov 2015)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 26504062
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Validation Study)
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Copyright | Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved. |
Chemical References |
- Biomarkers, Tumor
- Blood Proteins
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Topics |
- Biomarkers, Tumor
(blood)
- Blood Proteins
(analysis)
- Breast Neoplasms
(blood, diagnosis)
- Chromatography, Liquid
(methods)
- Female
- Humans
- Prognosis
- Proteomics
(methods)
- Spectrometry, Mass, Electrospray Ionization
(methods)
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