Effects of Vitamin K3 and K5 on Daunorubicin-resistant Human T Lymphoblastoid Leukemia Cells.

Abstract

BACKGROUND/AIM:

Anticancer efficacy of vitamin K derivatives on multidrug-resistant cancer cells has been scarcely investigated.

MATERIALS AND METHODS:

The effects of vitamins K3 and K5 on proliferation of human leukemia MOLT-4 cells and on daunorubicin-resistant MOLT-4/DNR cells were estimated by a WST assay. Apoptotic cells were detected by Annexin V and propidium iodide staining, followed by flow cytometry.

RESULTS:

Vitamins K3 and K5 significantly inhibited proliferation of leukemic cells at 10 and 100 μM (p<0.05), and these effects were almost equally observed in both MOLT-4 and MOLT/DNR drug-resistant cells. Vitamin K3 induced cell apoptosis at 10 and 100 μM in both MOLT-4 and MOLT-4/DNR cells (p<0.05). Vitamin K5 also increased apoptotic cells, while rather inducing necrotic cell death.

CONCLUSION:

Vitamins K3 and K5 suppress MOLT-4 and MOLT-4/DNR cell-proliferation partially through induction of apoptosis, and these vitamin derivatives can overcome drug resistance due to P-glycoprotein expression.

• Authors: Eri Nakaoka, Sachiko Tanaka, Kenji Onda, Kentaro Sugiyama, Toshihiko Hirano
• Journal: Anticancer research (Anticancer Res) Vol. 35 Issue 11 Pg. 6041-8 (Nov 2015) ISSN: 1791-7530 [Electronic] Greece
• PMID: 26504027 (Publication Type: Journal Article)
• Copyright: Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

Chemical References

• ATP Binding Cassette Transporter, Subfamily B, Member 1
• Antibiotics, Antineoplastic
• Vitamins
• Vitamin K 3
• vitamin k5
• Daunorubicin

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 1 (metabolism)
• Antibiotics, Antineoplastic (pharmacology)
• Apoptosis (drug effects)
• Cell Proliferation (drug effects)
• Daunorubicin (pharmacology)
• Drug Resistance, Multiple (drug effects)
• Drug Resistance, Neoplasm (drug effects)
• Flow Cytometry
• Humans
• Precursor T-Cell Lymphoblastic Leukemia-Lymphoma (drug therapy, metabolism, pathology)
• Tumor Cells, Cultured
• Vitamin K 3 (analogs & derivatives, pharmacology)
• Vitamins (pharmacology)