Theranostic etoposide phosphate/indium nanoparticles for cancer therapy and imaging.

Abstract

Etoposide phosphate (EP), a water-soluble anticancer prodrug, is widely used for treatment of many cancers. After administration it is rapidly converted to etoposide, its parent compound, which exhibits anticancer activity. Difficulty in parenteral administration necessitates the development of a suitable nanoparticle delivery system for EP. Here we have used indium both as a carrier to deliver etoposide phosphate to tumor cells and as a SPECT imaging agent through incorporation of (111)In. Etoposide phosphate was successfully encapsulated together with indium in nanoparticles, and exhibited dose dependent cytotoxicity and induction of apoptosis in cultured H460 cancer cells via G2/M cell cycle arrest. In a mouse xenograft lung cancer model, etoposide phosphate/indium nanoparticles induce tumor cell apoptosis, leading to significant enhancement of tumor growth inhibition compared to the free drug.

• Authors: Ramishetti Srinivas, Andrew Satterlee, Yuhua Wang, Yuan Zhang, Yongjun Wang, Leaf Huang
• Journal: Nanoscale (Nanoscale) Vol. 7 Issue 44 Pg. 18542-18551 (Nov 28 2015) ISSN: 2040-3372 [Electronic] England
• PMID: 26489694 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Drug Carriers
• Organophosphorus Compounds
• Indium
• etoposide phosphate
• Etoposide

Topics

• Animals
• Carcinoma, Non-Small-Cell Lung (drug therapy, metabolism, pathology)
• Drug Carriers
• Etoposide (analogs & derivatives, chemistry, pharmacology)
• G2 Phase Cell Cycle Checkpoints (drug effects)
• Humans
• Indium (chemistry, pharmacology)
• Lung Neoplasms (drug therapy, metabolism, pathology)
• M Phase Cell Cycle Checkpoints (drug effects)
• Mice
• Mice, Nude
• Nanoparticles (chemistry)
• Organophosphorus Compounds (chemistry, pharmacology)
• Theranostic Nanomedicine (methods)
• Xenograft Model Antitumor Assays