Abstract | BACKGROUND: Bael (Aegle marmelos (L.) Corr.) has been widely used in indigenous systems of Indian medicine to exploit its medicinal properties including astringent, antidiarrheal, antidysenteric, demulcent, antipyretic, antiulcer, anti-inflammatory and anti cancer activities. The present study aims to evaluate the antioxidative and antiulcer effect of methanolic extract of unripe fruit of Aegle marmelos (MEAM) against Helicobacter pylori-Lipopolysaccharide (HP-LPS) induced gastric ulcer in Sprague Dawley (SD) rats. METHODS: RESULTS:
Oral administration of HP-LPS (50 μg per animal) for four consecutive days resulted in induction of ulcer with the increase in gastric secretory parameters such as volume of gastric juice, free and total acidity, acid output, pepsin concentration. Oral administration of methanolic extract of Aegle marmelos fruit (MEAM) (25, 50, 100, 250 and 500 mg/kg) reduced the gastric ulcer by 2.8 %, 52.4 %, 73 %, 93 % and 93.98 %, respectively, compared to 89.2 % reduction by sucralfate (100 mg/kg). MEAM treatment significantly (p < 0.05) inhibited the increase in gastric secretory parameters in ulcerated rats, and it also prevented the reduction of enzymatic ( superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione transferase) and non-enzymatic antioxidants ( reduced glutathione, vitamin C and vitamin E) after HP-LPS induction. In addition, lipid peroxidation was inhibited by MEAM in HP-LPS induced rats. Results of histological analysis correlated well with biochemical parameters. CONCLUSION: These observations explored the antioxidant properties of MEAM contributing to the gastroprotective effect in HP-LPS induced gastric ulcer model.
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Authors | Yarasi Gayathri Ramakrishna, Kumarasamy Savithri, Manfred Kist, Sivasitamparam Niranjali Devaraj |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 15
Pg. 375
(Oct 19 2015)
ISSN: 1472-6882 [Electronic] England |
PMID | 26482072
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Lipopolysaccharides
- Plant Extracts
- lipopolysaccharide, Helicobacter pylori
- Catalase
- Superoxide Dismutase
- Glutathione Reductase
- Glutathione
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Topics |
- Aegle
(chemistry)
- Animals
- Catalase
(metabolism)
- Glutathione
(metabolism)
- Glutathione Reductase
(metabolism)
- Helicobacter Infections
(drug therapy, enzymology, metabolism, microbiology)
- Helicobacter pylori
(metabolism)
- Humans
- Lipid Peroxidation
(drug effects)
- Lipopolysaccharides
(metabolism)
- Male
- Oxidative Stress
(drug effects)
- Plant Extracts
(administration & dosage)
- Rats
- Rats, Sprague-Dawley
- Stomach Ulcer
(drug therapy, metabolism, microbiology)
- Superoxide Dismutase
(metabolism)
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