Abstract |
Epidemiological studies implicate environmental risk factors and epigenetic alterations in the multistage process of hepatocellular carcinoma (HCC) development. However, associations between environmental factors and DNA methylation of tumour suppressor genes (TSGs) in HCC development remain ambiguous. Understanding how possible interactions influence risk may provide insights into the complexity of hepato- carcinogenesis. For this study, blood samples were collected from HCC patients (n=90) and healthy volunteers (n=99) from Xiamen (China) and data for selected environmental risk factors [e.g., benzo[a]pyrene (B[a]P), hepatitis B or C virus (HBV or HCV) infection, smoking and alcohol consumption] were recorded; factors identified as significantly higher (P<0.05) amongst case subjects compared to controls were identified. In order to assess associations for epigenetic alterations and HCC risk factors, serum DNA methylation of TSGs was quantified using high-resolution melting (HRM) analysis. Our results clearly indicate elevated methylation patterns for detoxification gene [ glutathione-S-transferase Pi (GSTP)] promoter regions in cases compared to control subjects. Additionally, GSTP promoter hypermethylation and B[a]P diol epoxide- albumin ( BPDE-Alb) were positively correlated with HCC incidence. Our epidemiological and in vitro cell model studies indicated that GSTP promoter DNA methylation regulates this gene's expression. Moreover, GSTP also plays an important role in B[a]P detoxification and potential protective role against B[a]P-induced liver cell toxicity and hepato- carcinogenesis.
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Authors | Meiping Tian, Benhua Zhao, Jie Zhang, Francis L Martin, Qingyu Huang, Liangpo Liu, Heqing Shen |
Journal | The Science of the total environment
(Sci Total Environ)
Vol. 541
Pg. 1243-1252
(Jan 15 2016)
ISSN: 1879-1026 [Electronic] Netherlands |
PMID | 26476064
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier B.V. All rights reserved. |
Chemical References |
- Environmental Pollutants
- Benzo(a)pyrene
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Topics |
- Adult
- Benzo(a)pyrene
(toxicity)
- Carcinoma, Hepatocellular
(epidemiology, metabolism)
- Case-Control Studies
- China
(epidemiology)
- DNA Methylation
- Environmental Exposure
(statistics & numerical data)
- Environmental Pollutants
(toxicity)
- Female
- Humans
- Liver Neoplasms
(epidemiology, metabolism)
- Male
- Middle Aged
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