Abstract | AIM: MATERIALS & METHODS: The protein corona, cellular uptake mechanism and localization of Cu2O-NPs were investigated. Furthermore, the effect of Cu2O-NPs on uveal melanoma cell proliferation, migration and invasion, and possible mechanisms were studied in detail. RESULTS: Cu2O-NPs are able to adsorb serum proteins in cell culture medium, which are then internalized by uveal melanoma cells mainly through lipid raft-mediated endocytosis. Furthermore, Cu2O-NPs selectively inhibit cancer cell growth and impair the ability of uveal melanoma cell migration, invasion and the cytoskeleton assembly. The mechanism may be that Cu2O-NPs located in and damage mitochondria, autophagolysosomes and lysosomes, leading to elevated reactive oxygen species level and over-stimulated apoptosis and autophagy. CONCLUSION: The data provide detailed information of Cu2O-NPs for further application and indicate that Cu2O-NPs could be a potential agent for uveal melanoma therapy.
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Authors | Hongyuan Song, Qingqiang Xu, Yongzhe Zhu, Shiying Zhu, Hailin Tang, Yan Wang, Hao Ren, Ping Zhao, Zhongtian Qi, Shihong Zhao |
Journal | Nanomedicine (London, England)
(Nanomedicine (Lond))
Vol. 10
Issue 24
Pg. 3547-62
( 2015)
ISSN: 1748-6963 [Electronic] England |
PMID | 26467678
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Cell Proliferation
(drug effects)
- Copper
(administration & dosage, chemistry)
- Humans
- Melanoma
(drug therapy, pathology)
- Nanoparticles
(administration & dosage, chemistry)
- Neoplasm Invasiveness
(pathology)
- Uveal Neoplasms
(drug therapy, pathology)
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