Effects of Nephrolithiasis on Serum DNase (Deoxyribonuclease I and II) Activity and E3 SUMO-Protein Ligase NSE2 (NSMCE2) in Malaysian Individuals.

Abstract

OBJECTIVE:

Nephrolithiasis is one of the most common disorders of the urinary tract. The aim of this study was to examine a possible relationship between DNase I/II activity and E3 SUMO-protein ligase NSE2 in the sera of nephrolithiasis patients to evaluate the possibility of a new biomarker for evaluating kidney damage.

METHODS:

Sixty nephrolithiasis patients and 50 control patients were enrolled in a case-control study. Their blood urea, creatinine, protein levels and DNase I/II activity levels were measured by spectrometry. Serum NSMCE2 levels were measured by ELISA. Blood was collected from patients of the government health clinics in Kuantan-Pahang and fulfilled the inclusion criteria.

RESULTS:

The result indicated that mean levels of sera NSMCE2 have a significantly increase (P<0.01) in patients compared to control group. Compared with control subjects, activities and specific activities of serum DNase I and II were significantly elevated in nephrolithiasis patients (P$lt;0.01).

CONCLUSION:

This study suggests that an increase in serum concentrations of DNase I/II and E3 SUMO-protein ligase NSE2 level can be used as indicators for the diagnosis of kidney injury in patients with nephrolithiasis.

• Authors: Faridah Yusof, Atheer Awad Mehde, Wesen Adel Mehdi, Raha Ahmed Raus, Hamid Ghazali, Azlina Abd Rahman
• Journal: Biomedical and environmental sciences : BES (Biomed Environ Sci) Vol. 28 Issue 9 Pg. 660-5 (Sep 2015) ISSN: 0895-3988 [Print] China
• PMID: 26464253 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.

Chemical References

• Blood Proteins
• Hemoglobins
• Urea
• Creatinine
• Endodeoxyribonucleases
• Deoxyribonuclease I
• deoxyribonuclease II
• Ligases
• NSMCE2 protein, human

Topics

• Adult
• Blood Proteins (analysis)
• Case-Control Studies
• Creatinine (blood)
• Deoxyribonuclease I (blood)
• Endodeoxyribonucleases (blood)
• Hemoglobins (analysis)
• Humans
• Ligases (blood)
• Malaysia
• Middle Aged
• Nephrolithiasis (blood, enzymology)
• Urea (blood)