Abstract |
Previously, we showed that rice bran (RB) was able to reduce human rotavirus (HRV) diarrhea in gnotobiotic pigs. Here, we investigated its effect on the growth of diarrhea-reducing probiotic Lactobacillus rhamnosus GG (LGG) and Escherichia coli Nissle ( EcN), and the resulting effects on HRV diarrhea, gut epithelial health, permeability and innate immune responses during virulent HRV challenge. On 3, 5, and 7 days of age pigs were inoculated with 2 × 10(4) colony-forming-units LGG+EcN to initiate colonization. Daily RB supplementation (replacing 10% calorie intake) was started at 5 days of age and continued until euthanasia. A subset of pigs in each group was challenged orally with 10(5) focus-forming-units of virulent HRV at 33 days of age. RB completely prevented HRV diarrhea in LGG+EcN colonized pigs. RB significantly promoted the growth of both probiotic strains in the gut (~5 logs) and increased the body-weight-gain at 4-5 weeks of age compared to non-RB group. After HRV challenge, RB-fed pigs had significantly lower ileal mitotic index and villus width, and significantly increased intestinal IFN-γ and total IgA levels compared to non-RB group. Therefore, RB plus LGG+EcN colonization may represent a highly effective therapeutic approach against HRV and potentially a variety of other diarrhea-inducing enteric pathogens.
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Authors | Xingdong Yang, Erica Twitchell, Guohua Li, Ke Wen, Mariah Weiss, Jacob Kocher, Shaohua Lei, Ashwin Ramesh, Elizabeth P Ryan, Lijuan Yuan |
Journal | Scientific reports
(Sci Rep)
Vol. 5
Pg. 15004
(Oct 13 2015)
ISSN: 2045-2322 [Electronic] England |
PMID | 26459937
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Dietary Fiber
- Immunoglobulin A, Secretory
- Interferon-gamma
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Topics |
- Animals
- Body Weight
- Diarrhea
(immunology, therapy, virology)
- Dietary Fiber
(administration & dosage)
- Disease Models, Animal
- Humans
- Immunity, Innate
- Immunoglobulin A, Secretory
(immunology)
- Interferon-gamma
(metabolism)
- Intestinal Mucosa
(drug effects, immunology, microbiology, pathology, virology)
- Oryza
(chemistry)
- Permeability
- Probiotics
- Rotavirus
(physiology)
- Rotavirus Infections
(immunology, therapy, virology)
- Swine
- Virus Shedding
(drug effects)
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