Abstract | OBJECTIVE: MATERIALS AND METHODS: A549 cells were exposed for 24 hours to 300, 400, 500 or 600 µM of oleic acid. Cell morphological changes were observed using an inverted microscope, and cell proliferation was quantified with the Cell Counting Kit-8. Extracellular SP-B levels were assessed by ELISA, whereas intracellular SP-B expression by Western blot. RESULTS:
Oleic acid caused dose-dependent changes in cell morphology of A549 cells and decreased their proliferation. This was accompanied by release of SP-B into extracellular supernatants and corresponding decrease of intracellular levels of this protein. CONCLUSIONS:
Oleic acid causes a dose-dependent injury to A549 cells, release of SP-B into extracellular compartment, and decrease of intracellular SP-B expression. Our findings provide mechanistic insights into animal modeling of ALI with oleic acid.
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Authors | D-J Zhou, Y Chen, X-J Zhang, C Ma, J Qiu, J-H Zhou |
Journal | European review for medical and pharmacological sciences
(Eur Rev Med Pharmacol Sci)
Vol. 19
Issue 18
Pg. 3438-43
(Sep 2015)
ISSN: 2284-0729 [Electronic] Italy |
PMID | 26439040
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Pulmonary Surfactant-Associated Protein B
- Oleic Acid
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Topics |
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Dose-Response Relationship, Drug
- Enzyme-Linked Immunosorbent Assay
- Humans
- Lung Neoplasms
(drug therapy, metabolism, pathology)
- Oleic Acid
(pharmacology)
- Pulmonary Surfactant-Associated Protein B
(biosynthesis, genetics)
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