Abstract |
Colloidal silver nanoparticles (AgNPs) have attracted much attention in recent years as diagnostics and new drug delivery system in cancer medicine. To study the effects of plumbagin (PLB), a relatively non-toxic napthaquinone isolated from the roots of Plumbago indica in human cervical cancer cell line and developed a formulation to enhance its cytotoxic activities. Silver nanoparticles were synthesised by chemical reduction method and complexed with PLB. Both the AgNPs and the complex PLB-AgNPs were characterised by dynamic light scattering, high-resolution scanning electron microscopy and transmission electron microscopy. The amount of PLB and PLB-AgNPs internalised was determined by ultra-violet-visible spectrophotometer. Cell inhibition was determined by sulphorhodamine B assay. Mitotic index was determined by Wright-Giemsa staining. Apoptosis induction was assessed by western blot using cleaved poly adenosine diphosphate-ribose polymerase antibody. The scanning electron microscope analysis indicated an average particle size of 32±8 nm in diameter. Enhanced internalisation of PLB into the HeLa cells was observed in PLB-AgNPs. PLB inhibited proliferation of cells with IC50 value of about 18±0.6 µM and blocked the cells at mitosis in a concentration-dependent manner. PLB also inhibited the post- drug exposure clonogenic survival of cells and induced apoptosis. The antiproliferative, antimitotic and apoptotic activities were also found to be increased when cells were treated with PLB-AgNPs. The authors results support the idea that AgNP could be a promising and effective drug delivery system for enhanced activity of PLB in cancer treatment.
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Authors | Prakash Appadurai, Krishnan Rathinasamy |
Journal | IET nanobiotechnology
(IET Nanobiotechnol)
Vol. 9
Issue 5
Pg. 264-72
(Oct 2015)
ISSN: 1751-8741 [Print] United States |
PMID | 26435279
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Naphthoquinones
- Silver
- plumbagin
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Topics |
- Antineoplastic Agents
(chemistry, pharmacokinetics, pharmacology)
- Cell Proliferation
(drug effects)
- HeLa Cells
- Humans
- Metal Nanoparticles
(chemistry)
- Naphthoquinones
(chemistry, pharmacokinetics, pharmacology)
- Silver
(chemistry)
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