Abstract |
Pentraxin 3 (PTX3), a soluble pattern recognition receptor, plays an important role in innate immunity and has been implicated to be a candidate resistance gene against Streptococcus suis 2 infection. To discover the antibacterial effect of porcine PTX3 against S. suis 2, the 42-kDa PTX3 protein was expressed by Chinese hamster ovary cells (CHO), and an additional eukaryotic expression vector pVAX-ptx3 was constructed. The expressed porcine PTX3 mediated a range of antibacterial activities including increasing phagocytic capacity of primary porcine alveolar macrophages (PAM) against S. suis 2 and inhibiting adhesion of S. suis 2 to human epidermoid cancer cells (Hep-2). In mouse model, pre-intramuscular injecting with pVAX-ptx3 reduced mortality and reduced bacteria loads in blood, spleen, lung and brain compared with that of control group during 2-12 h following intraperitoneal injection (i.p.) with S. suis 2. Meanwhile, the expressions of IL-6 and IL-8 in blood were increased in pVAX-ptx3 group, whereas no obvious changes about IL-10. In piglet model, bacteria load in blood of pVAX-ptx3 group was significantly lower than that of control group after i.p. with S. suis 2, correspondingly, expression of IL-6 and IL-8 were significantly increased in pVAX-ptx3 group. In contrast, white blood cell (WBC) and neutrophil cell (NEU) count of peripheral blood in pVAX-ptx3 group were lower than that of control group. These studies described a novel antibacterial role for porcine PTX3 against S. suis 2 both in vitro and in vivo and suggested that porcine PTX3 may be a potential biological agent against S. suis 2 in pig and be used for the clinical prevention and treatment of streptococcosis caused by S. suis 2.
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Authors | Jueqiong Xu, Yanjuan Mu, Yue Zhang, Wenyang Dong, Yinchu Zhu, Jiale Ma, Wenchao Song, Zihao Pan, Chengping Lu, Huochun Yao |
Journal | Microbial pathogenesis
(Microb Pathog)
Vol. 89
Pg. 128-39
(Dec 2015)
ISSN: 1096-1208 [Electronic] England |
PMID | 26427882
(Publication Type: Journal Article)
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Copyright | Copyright © 2015 Elsevier Ltd. All rights reserved. |
Chemical References |
- Immunologic Factors
- Recombinant Proteins
- Serum Amyloid P-Component
- PTX3 protein
- C-Reactive Protein
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Topics |
- Animal Structures
(microbiology)
- Animals
- Bacterial Adhesion
(drug effects)
- Bacterial Load
- C-Reactive Protein
(administration & dosage, genetics, isolation & purification, pharmacology)
- Epithelial Cells
(microbiology)
- Immunologic Factors
(administration & dosage, pharmacology)
- Injections, Intramuscular
- Macrophages, Alveolar
(drug effects, immunology, microbiology)
- Mice
- Recombinant Proteins
(administration & dosage, genetics, isolation & purification, pharmacology)
- Serum Amyloid P-Component
(administration & dosage, genetics, isolation & purification, pharmacology)
- Streptococcal Infections
(microbiology, prevention & control)
- Streptococcus suis
(drug effects, immunology, physiology)
- Survival Analysis
- Swine
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