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Antibacterial effect of porcine PTX3 against Streptococcus suis type 2 infection.

Abstract
Pentraxin 3 (PTX3), a soluble pattern recognition receptor, plays an important role in innate immunity and has been implicated to be a candidate resistance gene against Streptococcus suis 2 infection. To discover the antibacterial effect of porcine PTX3 against S. suis 2, the 42-kDa PTX3 protein was expressed by Chinese hamster ovary cells (CHO), and an additional eukaryotic expression vector pVAX-ptx3 was constructed. The expressed porcine PTX3 mediated a range of antibacterial activities including increasing phagocytic capacity of primary porcine alveolar macrophages (PAM) against S. suis 2 and inhibiting adhesion of S. suis 2 to human epidermoid cancer cells (Hep-2). In mouse model, pre-intramuscular injecting with pVAX-ptx3 reduced mortality and reduced bacteria loads in blood, spleen, lung and brain compared with that of control group during 2-12 h following intraperitoneal injection (i.p.) with S. suis 2. Meanwhile, the expressions of IL-6 and IL-8 in blood were increased in pVAX-ptx3 group, whereas no obvious changes about IL-10. In piglet model, bacteria load in blood of pVAX-ptx3 group was significantly lower than that of control group after i.p. with S. suis 2, correspondingly, expression of IL-6 and IL-8 were significantly increased in pVAX-ptx3 group. In contrast, white blood cell (WBC) and neutrophil cell (NEU) count of peripheral blood in pVAX-ptx3 group were lower than that of control group. These studies described a novel antibacterial role for porcine PTX3 against S. suis 2 both in vitro and in vivo and suggested that porcine PTX3 may be a potential biological agent against S. suis 2 in pig and be used for the clinical prevention and treatment of streptococcosis caused by S. suis 2.
AuthorsJueqiong Xu, Yanjuan Mu, Yue Zhang, Wenyang Dong, Yinchu Zhu, Jiale Ma, Wenchao Song, Zihao Pan, Chengping Lu, Huochun Yao
JournalMicrobial pathogenesis (Microb Pathog) Vol. 89 Pg. 128-39 (Dec 2015) ISSN: 1096-1208 [Electronic] England
PMID26427882 (Publication Type: Journal Article)
CopyrightCopyright © 2015 Elsevier Ltd. All rights reserved.
Chemical References
  • Immunologic Factors
  • Recombinant Proteins
  • Serum Amyloid P-Component
  • PTX3 protein
  • C-Reactive Protein
Topics
  • Animal Structures (microbiology)
  • Animals
  • Bacterial Adhesion (drug effects)
  • Bacterial Load
  • C-Reactive Protein (administration & dosage, genetics, isolation & purification, pharmacology)
  • Epithelial Cells (microbiology)
  • Immunologic Factors (administration & dosage, pharmacology)
  • Injections, Intramuscular
  • Macrophages, Alveolar (drug effects, immunology, microbiology)
  • Mice
  • Recombinant Proteins (administration & dosage, genetics, isolation & purification, pharmacology)
  • Serum Amyloid P-Component (administration & dosage, genetics, isolation & purification, pharmacology)
  • Streptococcal Infections (microbiology, prevention & control)
  • Streptococcus suis (drug effects, immunology, physiology)
  • Survival Analysis
  • Swine

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