Identification of a conserved neutralizing linear B-cell epitope in the VP1 proteins of duck hepatitis A virus type 1 and 3.

Abstract

Duck virus hepatitis (DVH), mainly caused by duck hepatitis A virus (DHAV), is a severe disease threaten to duck industry and has worldwide distribution. As the major structural protein, the VP1 protein of DHAV is able to induce neutralizing antibody in ducks. In this study, a monoclonal antibody (mAb) 4F8 against the intact DHAV-1 particles was used to identify the possible epitope in the three serotypes of DHAV. The mAb 4F8 had weak neutralizing activities to both DHAV-1 and DHAV-3, and reacted with the conserved linear B-cell epitopes of (75)GEIILT(80) in DHAV-1 VP1 and (75)GEVILT(80) in DHAV-3 VP1 protein, respectively, while not with DHAV-2 VP1. This was the first report about identification of the common conserved neutralizing linear B-cell epitope of DHAV-1 and DHAV-3, which will facilitate understanding of the antigenic structure of VP1 and the serologic diagnosis of DHAV infection.

• Authors: Ruihua Zhang, Guomei Zhou, Yinghao Xin, Junhao Chen, Shaoli Lin, Ye Tian, Zhijing Xie, Shijin Jiang
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 180 Issue 3-4 Pg. 196-204 (Nov 18 2015) ISSN: 1873-2542 [Electronic] Netherlands
• PMID: 26427545 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies, Monoclonal
• Antibodies, Neutralizing
• Antigens, Viral
• Epitopes, B-Lymphocyte
• VP1 protein, hepatitis A virus
• Viral Structural Proteins

Topics

• Amino Acid Sequence
• Animals
• Antibodies, Monoclonal (immunology)
• Antibodies, Neutralizing (immunology)
• Antigens, Viral (chemistry, immunology)
• Ducks (virology)
• Epitope Mapping
• Epitopes, B-Lymphocyte (chemistry, immunology)
• Hepatitis Virus, Duck (immunology)
• Hepatitis, Viral, Animal (virology)
• Molecular Sequence Data
• Picornaviridae Infections (veterinary, virology)
• Poultry Diseases (virology)
• Viral Structural Proteins (chemistry, immunology)