Abstract | PURPOSE: The aim of this study was to improve the cytopathic effect (CPE) of antiamebic agents by combining with cellulose synthesis inhibitor as an encystation inhibitor. METHODS: RESULTS: 0.02% PHMB showed a 51.9% CPE on HCE cells within 30 minutes but exhibited significant toxic effects on Acanthamoeba. At a level of 0.00125%, PHMB had no significant CPEs on HCE cells, whereas 100 μM DCB and 10 μM isoxaben significantly inhibited the formation of the inner cyst wall of Acanthamoeba during encystation, and Acanthamoeba trophozoites failed to convert into mature cysts. Although a low concentration (0.00125%) of PHMB was used, the novel combinations with 100 μM DCB or 10 μM isoxaben had 23.4% or 18.7% additional amebicidal effects on Acanthamoeba. However, 100 μM DCB and 10 μM isoxaben had no CPEs on HCE cells. CONCLUSIONS: The combination of cellulose synthesis inhibitors with low concentrations of PHMB reduced the CPE on HCE cells and improved the amebicidal effect on Acanthamoeba by inhibition of encystation.
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Authors | Eun-Kyung Moon, Yeonchul Hong, Dong-Il Chung, Youn-Kyoung Goo, Hyun-Hee Kong |
Journal | Cornea
(Cornea)
Vol. 34
Issue 12
Pg. 1593-8
(Dec 2015)
ISSN: 1536-4798 [Electronic] United States |
PMID | 26426333
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amebicides
- Benzamides
- Biguanides
- Disinfectants
- Drug Combinations
- Nitriles
- polihexanide
- isoxaben
- Glucosyltransferases
- cellulose synthase
- dichlobanil
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Topics |
- Acanthamoeba Keratitis
(drug therapy, parasitology)
- Acanthamoeba castellanii
(drug effects, parasitology, ultrastructure)
- Amebicides
(toxicity)
- Animals
- Benzamides
(toxicity)
- Biguanides
(toxicity)
- Cells, Cultured
- Disinfectants
(toxicity)
- Drug Combinations
- Epithelium, Corneal
(parasitology)
- Eye Infections, Parasitic
(drug therapy, parasitology)
- Glucosyltransferases
(antagonists & inhibitors)
- Humans
- Nitriles
(toxicity)
- Parasite Encystment
(drug effects)
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