Efficient modification of CCR5 in primary human hematopoietic cells using a megaTAL nuclease and AAV donor template.

Abstract

Genetic mutations or engineered nucleases that disrupt the HIV co-receptor CCR5 block HIV infection of CD4(+) T cells. These findings have motivated the engineering of CCR5-specific nucleases for application as HIV therapies. The efficacy of this approach relies on efficient biallelic disruption of CCR5, and the ability to efficiently target sequences that confer HIV resistance to the CCR5 locus has the potential to further improve clinical outcomes. We used RNA-based nuclease expression paired with adeno-associated virus (AAV)-mediated delivery of a CCR5-targeting donor template to achieve highly efficient targeted recombination in primary human T cells. This method consistently achieved 8 to 60% rates of homology-directed recombination into the CCR5 locus in T cells, with over 80% of cells modified with an MND-GFP expression cassette exhibiting biallelic modification. MND-GFP-modified T cells maintained a diverse repertoire and engrafted in immune-deficient mice as efficiently as unmodified cells. Using this method, we integrated sequences coding chimeric antigen receptors (CARs) into the CCR5 locus, and the resulting targeted CAR T cells exhibited antitumor or anti-HIV activity. Alternatively, we introduced the C46 HIV fusion inhibitor, generating T cell populations with high rates of biallelic CCR5 disruption paired with potential protection from HIV with CXCR4 co-receptor tropism. Finally, this protocol was applied to adult human mobilized CD34(+) cells, resulting in 15 to 20% homologous gene targeting. Our results demonstrate that high-efficiency targeted integration is feasible in primary human hematopoietic cells and highlight the potential of gene editing to engineer T cell products with myriad functional properties.

• Authors: Blythe D Sather, Guillermo S Romano Ibarra, Karen Sommer, Gabrielle Curinga, Malika Hale, Iram F Khan, Swati Singh, Yumei Song, Kamila Gwiazda, Jaya Sahni, Jordan Jarjour, Alexander Astrakhan, Thor A Wagner, Andrew M Scharenberg, David J Rawlings
• Journal: Science translational medicine (Sci Transl Med) Vol. 7 Issue 307 Pg. 307ra156 (Sep 30 2015) ISSN: 1946-6242 [Electronic] United States
• PMID: 26424571 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2015, American Association for the Advancement of Science.

Chemical References

• Antigens, CD34
• CD3 Complex
• Receptors, Antigen, T-Cell
• Receptors, CCR5
• Green Fluorescent Proteins
• Deoxyribonucleases

Topics

• Adult
• Antigens, CD34 (metabolism)
• CD3 Complex (metabolism)
• Cells, Cultured
• DNA Repair
• Deoxyribonucleases (metabolism)
• Dependovirus (metabolism)
• Genetic Loci
• Genetic Therapy
• Green Fluorescent Proteins (metabolism)
• Hematopoietic Stem Cells (metabolism)
• Humans
• RNA Editing (genetics)
• Receptors, Antigen, T-Cell (metabolism)
• Receptors, CCR5 (metabolism)
• T-Lymphocytes (metabolism)