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miR-96 suppresses renal cell carcinoma invasion via downregulation of Ezrin expression.

AbstractBACKGROUND:
The present study examined the role of microRNA (miR)-96 in renal cell carcinoma (RCC) invasion.
METHODS:
The expression of miR-96 was detected by quantitative reverse transcription-polymerase chain reaction in human RCC cell lines with high (Caki-1) and low (786-O) metastatic potential. Invasive ability and Ezrin expression were assessed in Caki-1 and 786-O cells transfected with a miR-96 mimic or inhibitor using wound healing assays, Transwell assays and western blotting. Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months.
RESULTS:
miR-96 expression was significantly lower in Caki-1compared to786-O cells. The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic. Ezrin levels were negatively correlated with miR-96 in RCC, and inhibition of Ezrin expression suppressed the miR-96-induced change in invasive ability. The negative correlation between miR-96 and metastasis/Ezrin expression was also observed in human RCC specimens.
CONCLUSIONS:
These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.
AuthorsNengwang Yu, Shuai Fu, Yubao Liu, Zhonghua Xu, Yi Liu, Junwen Hao, Baocheng Wang, Aimin Zhang
JournalJournal of experimental & clinical cancer research : CR (J Exp Clin Cancer Res) Vol. 34 Pg. 107 (Sep 29 2015) ISSN: 1756-9966 [Electronic] England
PMID26419932 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Cytoskeletal Proteins
  • MIRN96 microRNA, human
  • MicroRNAs
  • ezrin
Topics
  • Carcinoma, Renal Cell (pathology)
  • Cell Line, Tumor
  • Cell Movement (genetics)
  • Cell Proliferation (genetics)
  • Cytoskeletal Proteins (biosynthesis)
  • Down-Regulation
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Kidney (pathology)
  • Kidney Neoplasms (pathology)
  • MicroRNAs (biosynthesis, genetics)
  • Neoplasm Invasiveness (genetics, pathology)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transfection

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