Abstract | BACKGROUND: METHODS: The expression of miR-96 was detected by quantitative reverse transcription-polymerase chain reaction in human RCC cell lines with high (Caki-1) and low (786-O) metastatic potential. Invasive ability and Ezrin expression were assessed in Caki-1 and 786-O cells transfected with a miR-96 mimic or inhibitor using wound healing assays, Transwell assays and western blotting. Expression of miR-96 and Ezrin was also examined in primary RCC samples from 17 patients with metastatic disease and 46 patients who maintained remission during a follow-up period of 37 months. RESULTS: miR-96 expression was significantly lower in Caki-1compared to786-O cells. The invasive ability of Caki-1 and 786-O cells increased following transfection of cells with miR-96 inhibitor, whereas it decreased following transfection with miR-96 mimic. Ezrin levels were negatively correlated with miR-96 in RCC, and inhibition of Ezrin expression suppressed the miR-96-induced change in invasive ability. The negative correlation between miR-96 and metastasis/ Ezrin expression was also observed in human RCC specimens. CONCLUSIONS: These results suggest that miR-96 suppresses RCC invasion by modulating Ezrin expression.
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Authors | Nengwang Yu, Shuai Fu, Yubao Liu, Zhonghua Xu, Yi Liu, Junwen Hao, Baocheng Wang, Aimin Zhang |
Journal | Journal of experimental & clinical cancer research : CR
(J Exp Clin Cancer Res)
Vol. 34
Pg. 107
(Sep 29 2015)
ISSN: 1756-9966 [Electronic] England |
PMID | 26419932
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytoskeletal Proteins
- MIRN96 microRNA, human
- MicroRNAs
- ezrin
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Topics |
- Carcinoma, Renal Cell
(pathology)
- Cell Line, Tumor
- Cell Movement
(genetics)
- Cell Proliferation
(genetics)
- Cytoskeletal Proteins
(biosynthesis)
- Down-Regulation
- Gene Expression Regulation, Neoplastic
- Humans
- Kidney
(pathology)
- Kidney Neoplasms
(pathology)
- MicroRNAs
(biosynthesis, genetics)
- Neoplasm Invasiveness
(genetics, pathology)
- Reverse Transcriptase Polymerase Chain Reaction
- Transfection
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