Abstract | AIMS/HYPOTHESIS: METHODS: Three vitamin D metabolism-related variants, DHCR7 rs12785878, CYP2R1 rs10741657 and GC rs2282679, were genotyped in 732 overweight/obese participants from a 2 year weight-loss trial (POUNDS Lost). We assessed genotype effects on changes in body weight, fasting levels of glucose and insulin, and HOMA-IR at 6 months (up to 656 participants) and 2 years (up to 596 participants) in response to low- protein vs high-protein diets, and low-fat vs high-fat diets. RESULTS: We found significant interactions between DHCR7 rs12785878 and diets varying in protein, but not in fat, on changes in insulin and HOMA-IR at both 6 months (p for interaction <0.001) and 2 years (p for interaction ≤ 0.03). The T allele ( vitamin-D-increasing allele) of DHCR7 rs12785878 was associated with greater decreases in insulin and HOMA-IR (p < 0.002) in response to high-protein diets, while there was no significant genotype effect on changes in these traits in the low-protein diet group. Generalised estimating equation analyses indicated significant genotype effects on trajectory of changes in insulin resistance over the 2 year intervention in response to high-protein diets (p < 0.001). We did not observe significant interaction between the other two variants and dietary protein or fat on changes in these traits. CONCLUSIONS/INTERPRETATION: TRIAL REGISTRATION: ClinicalTrials.gov NCT00072995.
|
Authors | Qibin Qi, Yan Zheng, Tao Huang, Jennifer Rood, George A Bray, Frank M Sacks, Lu Qi |
Journal | Diabetologia
(Diabetologia)
Vol. 58
Issue 12
Pg. 2791-9
(Dec 2015)
ISSN: 1432-0428 [Electronic] Germany |
PMID | 26416604
(Publication Type: Journal Article, Randomized Controlled Trial, Research Support, N.I.H., Extramural)
|
Chemical References |
- Blood Glucose
- Dietary Proteins
- Insulin
- Vitamin D
- Cytochrome P450 Family 2
- CYP2R1 protein, human
- Cholestanetriol 26-Monooxygenase
|
Topics |
- Adult
- Aged
- Blood Glucose
(metabolism)
- Body Weight
- Cholestanetriol 26-Monooxygenase
(genetics)
- Cytochrome P450 Family 2
- Diet, Protein-Restricted
- Dietary Proteins
(pharmacology)
- Female
- Genetic Variation
- Genotype
- Humans
- Insulin
(blood)
- Insulin Resistance
(genetics)
- Male
- Middle Aged
- Obesity
(diet therapy, genetics)
- Overweight
(diet therapy, genetics)
- Polymorphism, Single Nucleotide
- Treatment Outcome
- Vitamin D
(metabolism)
- Weight Loss
(genetics)
|