Neutrophil Gelatinase-Associated Lipocalin in Dogs With Sepsis Undergoing Emergency Laparotomy: A Prospective Case-Control Study.

Abstract

BACKGROUND:

Neutrophil gelatinase-associated lipocalin (NGAL) is an early indicator of acute kidney injury (AKI) in dogs and its use has not been evaluated in dogs with sepsis.

ANIMALS:

Fifteen dogs with sepsis requiring laparotomy (study dogs) and 10 dogs undergoing surgery for intervertebral disc disease (control dogs).

OBJECTIVE:

To determine whether NGAL increases in dogs with sepsis undergoing emergency laparotomy and whether it is correlated with development of AKI and survival.

METHODS:

Longitudinal study conducted at a referral teaching hospital. Serum neutrophil gelatinase-associated lipocalin (sNGAL), urinary NGAL normalized to urinary creatinine concentration (UNCR), and serum creatinine concentration were measured at 4 time points (admission, after anesthesia, and 24 and 48 hours postsurgery). Development of AKI (increase in serum creatinine concentration of 0.3 mg/dL) and in-hospital mortality were recorded. Linear mixed-model analysis was employed to assess differences between groups over time. Mann-Whitney U-test was performed for comparison of continuous variables between groups and Chi square or Fisher's exact tests were used to assess correlation between discrete data.

RESULTS:

Serum NGAL and UNCR were significantly higher in study dogs across all time points (P = .007 and P < .001, respectively) compared with controls. Urinary NGAL normalized to creatinine in the study group was not significantly different between survivors (n = 12) and nonsurvivors (n = 3). Dogs that received hydroxyethyl starch had significantly higher UNCR across all time points (P = .04) than those that did not.

DISCUSSION-CONCLUSION:

Serum neutrophil gelatinase-associated lipocalin and UNCR are increased in dogs with sepsis requiring emergency laparotomy. Additional studies are needed to evaluate its role as a marker of AKI in this population.

• Authors: S Cortellini, L Pelligand, H Syme, Y M Chang, S Adamantos
• Journal: Journal of veterinary internal medicine (J Vet Intern Med) 2015 Nov-Dec Vol. 29 Issue 6 Pg. 1595-602 ISSN: 1939-1676 [Electronic] United States
• PMID: 26415728 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.

Chemical References

• Acute-Phase Proteins
• Lipocalins
• Proto-Oncogene Proteins
• neutrophil gelatinase-associated lipocalin protein, dog

Topics

• Acute-Phase Proteins (genetics, metabolism)
• Animals
• Case-Control Studies
• Dog Diseases (metabolism, surgery)
• Dogs
• Emergencies
• Laparotomy (veterinary)
• Lipocalins (genetics, metabolism)
• Prospective Studies
• Proto-Oncogene Proteins (genetics, metabolism)
• Sepsis (metabolism, surgery, veterinary)