Abstract | BACKGROUND/AIMS: METHODS: The study population consisted of 80 female Wistar rats, divided in 3 groups, a group of prepubertal (Group A, n=30) and adult rats (Group B, n=20) that were androgenized via subcutaneous implantation of dihydrotestosterone-containing pellets as well as a group of adult non-androgenized rodents (Group C, n=30). All groups were randomly assigned either to a high-AGE or low-AGE diet for 3 months. RESULTS: Rats fed with a high-AGE diet exhibited significantly elevated levels of gamma-glutamyl transferase (x03B3;GT) (p≤0.0002) and indices of AGE immunostaining in liver tissue (p<0.01) when compared to the respective low-AGE group, while aspartate aminotransferase (AST) levels were affected only in non-androgenized animals (p=0.0002). Androgenization per se constitutes an aggravating factor as demonstrated by the elevated x03B3;GT levels in adult androgenized animals compared to non-androgenized, independent of diet content (p=0.0002) and by the elevated AST and alanine aminotransferase (ALT) levels in low-AGE subgroups (adult androgenized vs. non-androgenized, p=0.0002) followed by increased immunohistochemical AGE deposition in hepatocytes of the latter categories (p=0.0007). CONCLUSION: The present study suggests that androgens and glycotoxins may contribute synergistically to distort hepatic physiology and function as observed in hyperandrogenic conditions.
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Authors | Eleni Palioura, Sotiria Palimeri, Christina Piperi, Stratigoula Sakellariou, Eleni Kandaraki, Theodoros Sergentanis, Georgia Levidou, George Agrogiannis, Apostolos Papalois, Penelope Korkolopoulou, Evanthia Diamanti-Kandarakis, Athanasios G Papavassiliou |
Journal | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
(Cell Physiol Biochem)
Vol. 37
Issue 3
Pg. 1134-46
( 2015)
ISSN: 1421-9778 [Electronic] Germany |
PMID | 26414164
(Publication Type: Journal Article)
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Copyright | © 2015 The Author(s) Published by S. Karger AG, Basel. |
Chemical References |
- Androgens
- Glycation End Products, Advanced
- gamma-Glutamyltransferase
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Topics |
- Androgens
(adverse effects, pharmacology)
- Animals
- Dietary Supplements
- Disease Models, Animal
- Female
- Glycation End Products, Advanced
(adverse effects, pharmacology)
- Humans
- Liver
(drug effects, metabolism)
- Polycystic Ovary Syndrome
(chemically induced, enzymology)
- Rats
- Rats, Wistar
- gamma-Glutamyltransferase
(metabolism)
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