Epigenetic silencing of a long non-coding RNA KIAA0495 in multiple myeloma.

Abstract

In multiple myeloma, a long non-coding RNA, KIAA0495 (alias PDAM/TP73-AS1), had been found progressively downregulated from normal plasma cell to benign monoclonal gammopathy of undetermined significance to symptomatic myeloma. Herein, by methylation-specific PCR, the putative KIAA0495 promoter was found unmethylated in all healthy controls (N = 14) but methylated in 50 % of myeloma cell lines (N = 10). KIAA0495 methylation was shown inversely correlated with KIAA0495 expression. However, KIAA0495 methylation was detected in none of both primary myeloma samples at diagnosis (N = 61) and at relapse/progression (N = 16). Collectively, despite frequently methylated in cell lines, KIAA0495 methylation appeared unimportant in the pathogenesis or progression of myeloma.

• Authors: Kwan Yeung Wong, Zhenhai Li, Xiaoqin Zhang, Gilberto Ka Kit Leung, Godfrey Chi-Fung Chan, Chor Sang Chim
• Journal: Molecular cancer (Mol Cancer) Vol. 14 Pg. 175 (Sep 26 2015) ISSN: 1476-4598 [Electronic] England
• PMID: 26410378 (Publication Type: Journal Article)

Chemical References

• RNA, Long Noncoding
• long non-coding RNA KIAA0495, human

Topics

• Adult
• Aged
• Aged, 80 and over
• DNA Methylation (genetics)
• Disease Progression
• Epigenesis, Genetic
• Female
• Gene Expression Regulation, Neoplastic
• Gene Silencing
• Humans
• Male
• Middle Aged
• Multiple Myeloma (genetics, pathology)
• RNA, Long Noncoding (antagonists & inhibitors, genetics)