Abstract | OBJECTIVE: The role of miR-365 in cancer cells seemed controversial in previous studies. We thereby in this article aimed to define the role of miR-365 in malignant melanoma (MM) pathogenesis. METHODS: RESULTS: MiR-365 was strongly down-regulated in malignant melanoma (MM) tissues and cell lines, and its expression levels were associated with lymph node metastasis and clinical stage, as well as overall survival and replase-free survival of MM. We also found that ectopic expression of miR-365 inhibited MM cell proliferation and MM metastasis in vitro and in vivo. We further identified a novel mechanism of miR-365 to suppress MM growth and metastasis. NRP1 was proved to be a direct target of miR-365, using luciferase assay and western blot. NRP1 over-expression in miR-365 expressing cells could rescue invasion and growth defects of miR-365. In addition, miR-365 expression inversely correlated with NRP1 protein levels in MM. CONCLUSION: Our data suggest that miR-365 functions as a tumor suppressor in MM development and progression, and holds promise as a prognostic biomarker and potential therapeutic target for MM.
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Authors | Juanjuan Bai, Zhongling Zhang, Xing Li, Huifan Liu |
Journal | Cancer biomarkers : section A of Disease markers
(Cancer Biomark)
Vol. 15
Issue 5
Pg. 599-608
( 2015)
ISSN: 1875-8592 [Electronic] Netherlands |
PMID | 26406949
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- MIRN365 microRNA, human
- MicroRNAs
- Neuropilin-1
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Topics |
- Animals
- Biomarkers, Tumor
(biosynthesis, genetics)
- Cell Proliferation
(genetics)
- Disease-Free Survival
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Kaplan-Meier Estimate
- Lymphatic Metastasis
- Male
- Melanoma
(genetics, pathology)
- Mice
- MicroRNAs
(biosynthesis, genetics)
- Neoplasm Invasiveness
(genetics)
- Neoplasm Metastasis
- Neuropilin-1
(biosynthesis, genetics)
- Xenograft Model Antitumor Assays
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