Abstract | RATIONALE: METHODS: LS174T colorectal adenocarcinoma xenografts grown in male immune-deficient mice were treated with 27.5 mg/kg DMXAA. The control (before treatment) and 4 h and 24 h post-treatment tumours were excised and divided into two. MALDI-MS imaging experiments were carried out on 12 µm cryosections sections taken from one half of the tumours and from the other half the lipids were extracted and analysed by TLC/MALDI-MS. These experiments were carried out in triplicate. RESULTS: Statistical analysis of the MALDI-MS imaging data set indicated an increased amount of LPC in the 24 h post-treated sample and a decreased amount of PC in the 24 h post-treated sample, compared with the 4 h post-treated sample and the control. These effects were confirmed by the TLC/MALDI-MS data. The lipid extracts were separated into six spots on the TLC plate. These were identified as arising from different lipids classes, i.e. LPC, sphingomyelins (SM), phosphatidylcholines (PC) and phosphatidylethanolamines (PE). The TLC/MALDI-MS data indicated that LPC were highly expressed in the 4 h and 24 h post-treated tumour samples compared with the control. Examination of the mass spectrometric images confirms this increase and demonstrates additionally that the increase in the signals arising from LPC appears to be localised primarily within the central areas of the xenograft. CONCLUSIONS: An increase in expression of LPC lipids in solid tumours treated with DMXAA has been demonstrated and shown to be localised in the central area of the tumour.
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Authors | Afnan Batubara, Vikki A Carolan, Paul M Loadman, Chris Sutton, Steve D Shnyder, Malcolm R Clench |
Journal | Rapid communications in mass spectrometry : RCM
(Rapid Commun Mass Spectrom)
Vol. 29
Issue 14
Pg. 1288-96
(Jul 30 2015)
ISSN: 1097-0231 [Electronic] England |
PMID | 26405790
(Publication Type: Journal Article)
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Copyright | Copyright © 2015 John Wiley & Sons, Ltd. |
Chemical References |
- Antineoplastic Agents
- Phospholipids
- Xanthones
- vadimezan
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Topics |
- Adenocarcinoma
(drug therapy, pathology)
- Animals
- Antineoplastic Agents
(therapeutic use)
- Chromatography, Thin Layer
(methods)
- Colon
(drug effects, pathology)
- Colorectal Neoplasms
(drug therapy, pathology)
- Male
- Mice
- Mice, Nude
- Phospholipids
(analysis)
- Rectum
(drug effects, pathology)
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
(methods)
- Xanthones
(therapeutic use)
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