Rutin Increases Muscle Mitochondrial Biogenesis with AMPK Activation in High-Fat Diet-Induced Obese Rats.

Abstract

Decreased mitochondrial number and dysfunction in skeletal muscle are associated with obesity and the progression of obesity-associated metabolic disorders. The specific aim of the current study was to investigate the effects of rutin on mitochondrial biogenesis in skeletal muscle of high-fat diet-induced obese rats. Supplementation with rutin reduced body weight and adipose tissue mass, despite equivalent energy intake (p < 0.05). Rutin significantly increased mitochondrial size and mitochondrial DNA (mtDNA) content as well as gene expression related to mitochondrial biogenesis, such as peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), nuclear respiratory factor-1 (NRF-1), transcription factor A (Tfam), and nicotinamide adenine dinucleotide (NAD)-dependent deacetylase, sirtulin1 (SIRT1) in skeletal muscle (p < 0.05). Moreover, rutin consumption increased muscle adenosine monophosphate-activated protein kinase (AMPK) activity by 40% (p < 0.05). Taken together, these results suggested at least partial involvement of muscle mitochondria and AMPK activation in the rutin-mediated beneficial effect on obesity.

• Authors: Sangjin Seo, Mak-Soon Lee, Eugene Chang, Yoonjin Shin, Soojung Oh, In-Hwan Kim, Yangha Kim
• Journal: Nutrients (Nutrients) Vol. 7 Issue 9 Pg. 8152-69 (Sep 22 2015) ISSN: 2072-6643 [Electronic] Switzerland
• PMID: 26402699 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Obesity Agents
• DNA, Mitochondrial
• Nuclear Respiratory Factor 1
• Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
• Ppargc1a protein, rat
• Tfam protein, rat
• Transcription Factors
• Rutin
• AMP-Activated Protein Kinases
• Sirt1 protein, rat
• Sirtuin 1

Topics

• AMP-Activated Protein Kinases (metabolism)
• Adiposity (drug effects)
• Animals
• Anti-Obesity Agents (pharmacology)
• DNA, Mitochondrial (metabolism)
• Diet, High-Fat
• Disease Models, Animal
• Enzyme Activation
• Male
• Mitochondria, Muscle (drug effects, enzymology)
• Mitochondrial Size (drug effects)
• Muscle, Skeletal (drug effects, enzymology)
• Nuclear Respiratory Factor 1 (genetics, metabolism)
• Obesity (drug therapy, enzymology, genetics, physiopathology)
• Organelle Biogenesis
• Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
• Rats, Sprague-Dawley
• Rutin (pharmacology)
• Signal Transduction (drug effects)
• Sirtuin 1 (genetics, metabolism)
• Time Factors
• Transcription Factors (genetics, metabolism)
• Weight Loss (drug effects)