Abstract |
Two RNA fragments linked by means of a 2',5' phosphodiester bridge (2' hydroxyl of one fragment connected to the 5' hydroxyl of the other) constitute a class of nucleic acids known as 2'-5' branched RNAs (bRNAs). In this report we show that bRNA analogues containing 2'-5' phosphoramidate linkages (bN-RNAs) inhibit the lariat debranching enzyme, a 2',5'-phosphodiesterase that has recently been implicated in neurodegenerative diseases associated with aging. bN-RNAs were efficiently generated using automated solid-phase synthesis and suitably protected branchpoint building blocks. Two orthogonally removable groups, namely the 4-monomethoxytrityl (MMTr) group and the fluorenylmethyl-oxycarbonyl (Fmoc) groups, were evaluated as protecting groups of the 2' amino functionality. The 2'-N-Fmoc methodology was found to successfully produce bN-RNAs on solid-phase oligonucleotide synthesis. The synthesized bN-RNAs resisted hydrolysis by the lariat debranching enzyme (Dbr1) and, in addition, were shown to attenuate the Dbr1-mediated hydrolysis of native bRNA.
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Authors | Nobuhiro Tago, Adam Katolik, Nathaniel E Clark, Eric J Montemayor, Kohji Seio, Mitsuo Sekine, P John Hart, Masad J Damha |
Journal | The Journal of organic chemistry
(J Org Chem)
Vol. 80
Issue 20
Pg. 10108-18
(Oct 16 2015)
ISSN: 1520-6904 [Electronic] United States |
PMID | 26378468
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Amides
- Phosphoric Acids
- RNA
- phosphoramidic acid
- RNA Nucleotidyltransferases
- lariat debranching enzyme
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Topics |
- Amides
(chemistry)
- Humans
- Nucleic Acid Conformation
- Phosphoric Acids
(chemistry)
- RNA
(chemical synthesis, chemistry, metabolism)
- RNA Nucleotidyltransferases
(antagonists & inhibitors, chemistry)
- RNA Splicing
- Solid-Phase Synthesis Techniques
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