Abstract | BACKGROUND: METHODS: The nociception was assessed by measuring the incidence of foot withdrawal in response to mechanical indentation in rats (n = 8). Cytokine expression was measured using quantitative polymerase chain reaction (n = 8). Cell signalings were assayed by western blot (n = 4) and immunohistochemistry (n = 5). The microglial cell line BV-2, primary astrocytes, and neuron-like SH-SY5Y cells were cultured to investigate the in vitro effects. RESULTS: CONCLUSIONS: AMPK activation in the spinal glia by resveratrol may have utility in the treatment of TCI-induced neuroinflammation, and our results further implicate AMPK as a novel target for the attenuation of bone cancer pain.
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Authors | Huayuan Song, Yuan Han, Cailong Pan, Xueting Deng, Wenling Dai, Liang Hu, Chunyi Jiang, Yanjing Yang, Zhixiang Cheng, Fei Li, Guangqin Zhang, Xuefeng Wu, Wentao Liu |
Journal | Anesthesiology
(Anesthesiology)
Vol. 123
Issue 5
Pg. 1170-85
(Nov 2015)
ISSN: 1528-1175 [Electronic] United States |
PMID | 26378398
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Protein Kinase Inhibitors
- Stilbenes
- Mitogen-Activated Protein Kinases
- AMP-Activated Protein Kinases
- Resveratrol
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Topics |
- AMP-Activated Protein Kinases
(metabolism)
- Animals
- Bone Neoplasms
(drug therapy, enzymology, pathology)
- Cell Line, Tumor
- Female
- Humans
- Inflammation
(enzymology, prevention & control)
- Mitogen-Activated Protein Kinases
(antagonists & inhibitors, metabolism)
- Pain
(enzymology, pathology, prevention & control)
- Protein Kinase Inhibitors
(pharmacology, therapeutic use)
- Rats
- Rats, Sprague-Dawley
- Resveratrol
- Stilbenes
(pharmacology, therapeutic use)
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